Quant 3 pcr system

Manufactured by Thermo Fisher Scientific

The Quant 3 PCR system is a real-time PCR (qPCR) instrument designed for accurate and reliable nucleic acid quantification. It features a compact and user-friendly design, providing consistent performance for a wide range of real-time PCR applications.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (3 mentions) Power sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions) Mm01228299 m1, by Thermo Fisher Scientific (1 mentions) Qiashredder, by Qiagen (1 mentions) Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions) Biomasher, by Takara Bio (1 mentions) Taqman gene expression assay kit, by Thermo Fisher Scientific (1 mentions) Accupower rt premix, by Bioneer (1 mentions) Accuprep universal rna extraction kit, by Bioneer (1 mentions) Sybr green master mix, by Thermo Fisher Scientific (1 mentions)

4 protocols using quant 3 pcr system

RAW264.7 Cytokine Expression Assay

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RAW264.7 cells were seeded into a 6-well plate (2 × 10⁶ cells/well) and cultured for 24 h. The cells were treated with coumarin derivative 2 at 20, 40, and 80 μM and cultured for 2 h and with LPS (500 ng/mL) for 6 h.
The cells were washed with phosphate-buffered saline (PBS) and lysed with the RNeasy Mini kit (Qiagen, Hilden, Germany) for RNA isolation. RNA was converted to cDNA using the AccuPower reverse transcriptase Premix Kit (Bioneer, Daejeon, Republic of Korea). A Power SYBR Green PCR Master Mix (Applied Biosystems, Waltham, MN, USA) was used to perform polymerase chain reaction amplification. Amplification conditions were selected within the Quant 3 PCR system (Applied Biosystems). The data were normalized to the amount of glyceraldehyde 3-phosphate dehydrogenase.

Min S.J., Lee H., Shin M.S, & Lee J.W. (2023). Synthesis and Biological Properties of Pyranocoumarin Derivatives as Potent Anti-Inflammatory Agents. International Journal of Molecular Sciences, 24(12), 10026.

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Quantifying Inflammatory Markers in RAW264.7 Cells

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RAW264.7 cells were plated into a 6-well plate (2 × 10⁶ cells/well) and then treated with 3-(3-hydroxyphenyl)-indolin-2-one for 2 h, followed by treatment with LPS (500 ng/mL) for 6 h (for IL-6, TNF-α, and iNOS mRNA expression). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, CA, USA), and cDNA was synthesized using the AccuPower revese transcriptase Premix Kit (Bioneer, Daejon, Republic of Korea). qRT-PCR quantification was analyzed using TaqMan Fast Advanced Master mix with iNOS (Mm00440502_m1), IL-6 (Mm00446190_m1), TNF-α (Mm00443258_m1) and GAPDH (Mm99999915_g1) TaqMan primer sets (Applied Biosystems, CA, USA). Amplification conditions were determined by the Quant 3 PCR system (Applied Biosystems).

Kim S.J., Lee S.H., Lee H., Shin M.S, & Lee J.W. (2023). Design, Synthesis, and Biological Evaluation of 3-Substituted-Indolin-2-One Derivatives as Potent Anti-Inflammatory Agents. International Journal of Molecular Sciences, 24(3), 2066.

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Intestinal Defensins, Lysozyme, and Claudin Expression

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Mouse small intestines tissues were extracted using BioMasher (TaKaRa, Shiga, Japan) and filtrated using a QIAshredder (Qiagen, Hilden, Germany). Total RNA from the intestinal tissues was isolated and purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), followed by cDNA synthesis using the RevertAid First-Strand cDNA synthesis kit (Fermentas, MA, USA). The qRT-PCR quantification was performed using Mm02524428_g1(α-defensin-1), Mm01228299_m1 (lysozyme), and Mm01342184_m1 (claudin1) TaqMan primer sets (Applied Biosystems, Foster City, CA, USA). The amplification conditions were determined using the Quant 3 PCR system (Applied Biosystems).

Kim S.J., Shin M.S, & Choi Y.K. (2024). Ameliorative Effects of Zingiber officinale Rosc on Antibiotic-Associated Diarrhea and Improvement in Intestinal Function. Molecules, 29(3), 732.

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mRNA Expression Analysis of EMT Markers

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For mRNA expression analysis, A549 cells were seeded at a density of 5 × 10⁵ cells/well in a six-well plate and incubated overnight. After changing the medium to serum-free medium, cells were treated with GT at concentrations of 50, 75, and 100 μg/mL for 6 h, followed by treatment with TGF-β1 (5 ng/mL) for 18 h. Total RNA was extracted using an AccuPrep Universal RNA Extraction Kit (Bioneer, Daejeon, Republic of Korea) and reverse-transcribed using an AccuPower RT premix (Bioneer). Real-time PCR was performed using the TaqMan gene expression assay kit (Applied Biosystems, Foster City, CA, USA) or SYBR Green Master Mix (Applied Biosystems), with specific primers for E-cad (sense and anti-sense primers 5′-CCACCAAAGTCACGCTGAATAC-3′ and 5′ GAAGAAGAGGACAGCACTG-3′), Vim (Hs_00958111_m1), N-cad (Hs_00983056_m1), ZEB1 (Hs_01566408_m1), Twist (Hs00361186_m1), and GAPDH (Hs_02786624_m1). qPCR results were determined from triplicate reactions. mRNA levels were determined by the Quant 3 PCR system (Applied Biosystems).

Kim S.J., Nah S.Y., Park I.H., Shin M.S, & Kang K.S. (2023). Gintonin Isolated from Ginseng Inhibits the Epithelial—Mesenchymal Transition Induced by TGF-β in A549 Lung Cancer Cells. Plants, 12(10), 2013.

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