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Spinning disc confocal tirf fura microscope

Manufactured by Zeiss
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The Spinning disc confocal & TIRF Fura microscope is a lab equipment designed to perform optical microscopy. It combines spinning disc confocal and total internal reflection fluorescence (TIRF) imaging techniques, as well as Fura-2 calcium imaging capabilities. The core function of this microscope is to provide high-resolution, real-time imaging of live cell samples.

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Lab products found in correlation

Ph sensitive lysosensor yellow blue dnd 160, by Thermo Fisher Scientific (2 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Confocal microscope (860 citations) Spinning disc confocal microscope (41 citations) TIRF microscope (12 citations) Spinning disc microscope (10 citations)

2 protocols using spinning disc confocal tirf fura microscope

1

Lysosome Visualization in Live Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were treated with the respective molecules in 2-well chambered coverglass slides at a final concentration of 1 μM for 18 hours followed by addition of lysosome-specific dyes. LysoTracker™ Red DND-99 and pH sensitive-LysoSensor™ Yellow/Blue DND-160 purchased from ThermoFisher were added to the cells for 1 hour at 37° C along with Hoescht to stain DNA according to the manufacturer’s recommendations. Live cell images were captured at 20X with a Zeiss spinning disc confocal & TIRF Fura microscope at UCSF’s Laboratory for Cell Analysis.
Tolani B., Celli A., Yao Y., Tan Y.Z., Fetter R., Liem C.R., de Smith A.J., Vasanthakumar T., Bisignano P., Cotton A.D., Seiple I.B., Rubinstein J.L., Jost M, & Weissman J.S. (2022). Ras-mutant cancers are sensitive to small molecule inhibition of V-type ATPases in mice. Nature Biotechnology, 40(12), 1834-1844.
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2

Lysosome Imaging in Live Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were treated with the respective molecules in 2-well chambered coverglass slides at a final concentration of 1 µM for 18 hours followed by addition of lysosome-specific dyes. LysoTracker™ Red DND-99 and pH sensitive-LysoSensor™ Yellow/Blue DND-160 purchased from ThermoFisher were added to the cells for 1 hour at 37º C along with Hoescht to stain DNA according to the manufacturer's recommendations. Live cell images were captured at 20X with a Zeiss spinning disc confocal & TIRF Fura microscope at UCSF's Laboratory for Cell Analysis.
Tolani B., Celli A., Yao Y., Tan Y.Z., Fetter R.D., Liem C.R., Smith A.J.d., Vasanthakumar T., Bisignano P., Seiple I.B., Rubinstein J.L., Jost M., & Weissman J.S. (2020). Selective Targeting of Ras Mutant Cancers via a New Small Molecule.
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