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Lps derived from e coli 0111 b4 l2630

Manufactured by Merck Group

LPS derived from E. coli 0111:B4 (L2630) is a laboratory reagent. It is a purified lipopolysaccharide extracted from the Gram-negative bacterium Escherichia coli, strain 0111:B4.

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2 protocols using lps derived from e coli 0111 b4 l2630

1

Immunoblotting and Immunostaining Analysis of Lysosomal Regulators

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LPS derived from E. coli 0111:B4 (L2630) and RIPA buffer (R0278) were purchased from Sigma (St. Louis, MO), Luria-Bertani broth (L8050) Luria-Bertani Agar+50μg/ml Ampicillin (L1002) were from Teknova (Hollister, CA). PBS (10010–023) was obtained from Thermo Fisher Scientific (Waltham, MA), whereas saline (MSDW-1000) was from Growcells (Irvine, CA). Western blot analysis was conducted using specific antibodies to LAMP1 (Ab25245; Abcam Cambridge, MA), TFEB (A303–673A; Bethyl, Montgomery, TX), and ZKSCAN3 (SC515285; Santa Cruz, Dallas TX). Antibodies to TFE3 (HPA023881), LC3 (L8918) and β-actin (A5441) were from Sigma (St. Louis, MO). Antibodies for immunohistochemistry are as the follows: Rubicon: Proteintech 21444–1-AP.
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2

Immunoblotting and Immunostaining Analysis of Lysosomal Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
LPS derived from E. coli 0111:B4 (L2630) and RIPA buffer (R0278) were purchased from Sigma (St. Louis, MO), Luria-Bertani broth (L8050) Luria-Bertani Agar+50μg/ml Ampicillin (L1002) were from Teknova (Hollister, CA). PBS (10010–023) was obtained from Thermo Fisher Scientific (Waltham, MA), whereas saline (MSDW-1000) was from Growcells (Irvine, CA). Western blot analysis was conducted using specific antibodies to LAMP1 (Ab25245; Abcam Cambridge, MA), TFEB (A303–673A; Bethyl, Montgomery, TX), and ZKSCAN3 (SC515285; Santa Cruz, Dallas TX). Antibodies to TFE3 (HPA023881), LC3 (L8918) and β-actin (A5441) were from Sigma (St. Louis, MO). Antibodies for immunohistochemistry are as the follows: Rubicon: Proteintech 21444–1-AP.
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