Vt env 414s

Manufactured by Med Associates

Sourced in Georgia

The VT-ENV-414S is a laboratory equipment product. It is designed to provide controlled environmental conditions for various experimental or testing purposes. The core function of this product is to maintain and regulate temperature, humidity, and other environmental parameters within a specific range.

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Freezeframe 3, by Actimetrics (3 mentions)

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ENV-414S (22 citations)

3 protocols using vt env 414s

Fear Conditioning in Plexiglas Chambers

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The apparatus and stimuli used have been previously described (Heroux et al. 2016 (link); Murawski and Stanton 2010 (link); Murawski et al., 2012 (link); Robinson-Drummer et al. 2016 (link)). Fear conditioning occurred in four Plexiglas chambers measuring 16.5 cm × 12.1 cm × 21.6 cm which were arranged in a 2 × 2 formation on a Plexiglas stand within a fume hood to provide ambient light and background noise (Context A). Each chamber had a grid floor made of nine stainless steel bars (11.5 cm from the top of the chamber), 0.5 cm in diameter and spaced 1.25 cm apart. The alternate context (Context B) consisted of the same Plexiglas chambers with a convex wire mesh insert that covered the back wall and floor of the chamber and a white paper sleeve that covered the outside walls of the chamber. The 2-sec, 1.5-mA footshock unconditioned stimulus (US) was delivered using a shock scrambler (VT ENV-414S, Med Associates) connected to the grid floor of the chamber. The fear chambers were cleaned with 5% ammonium hydroxide solution prior to each load of experimental animals. Videos of each session (preexposure, training, testing) were recorded using Freeze Frame 3.0 software (Actimetrics) with freezing defined as a bout of 0.75 sec or longer without a change in video pixilation.

Heroux N.A., Robinson-Drummer P.A., Sanders H.R., Rosen J.B, & Stanton M.E. (2017). Differential involvement of the medial prefrontal cortex across variants of contextual fear conditioning. Learning & Memory, 24(8), 322-330.

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Contextual Fear Conditioning Apparatus

Cited 1 time

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The apparatus and stimuli used have been described previously [37 (link),46 (link)]. Briefly, fear conditioning occurred in four clear Plexiglas chambers arranged in a 2 × 2 formation on a Plexiglas stand within a fume hood (Context A – Pre Group). Each chamber had a grid floor made of nine stainless steel bars connected to a shock scrambler (Med Associates, Georgia, VT-ENV-414S). The alternate context (Context B – Alt-Pre Group) consisted of the same Plexiglas chambers with a convex wire mesh insert that covered the back wall and floor of the chamber and a white paper sleeve that covered the outside walls of the chamber. In Experiment 1B, 2, and 3, the unconditioned stimulus (US) was two, 1.5 mA foot-shocks, each 2s in duration, and presented 1s apart immediately upon chamber entry. In Experiment 1A, one immediate shock was given instead of two. Videos of each session (preexposure, training, testing) were recorded using Freeze-Frame 3.0 software (Actimetrics, Wilmette IL) with freezing defined as a bout of 0.75 s or longer without a change in video pixilation (see section 2.8.2).

Heroux N.A., Robinson-Drummer P.A., Kawan M., Rosen J.B, & Stanton M.E. (2018). Neonatal ethanol exposure impairs long-term context memory formation and prefrontal immediate early gene expression in rats. Behavioural brain research, 359, 386-395.

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Fear Conditioning Apparatus and Protocols

Cited 1 time

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The apparatus and stimuli used have been described previously [55 (link),60 ,63 (link)]. Briefly, fear conditioning occurred in four clear Plexiglas chambers within a fume hood (Context A – Pre group), with grid floors connected to shock scramblers (Med Associates, Georgia, VT-ENV-414S). The alternate context (Context B – Alt-Pre group) consisted of the same Plexiglas chambers with a convex wire mesh insert that covered the back wall and floor of the chamber and a white paper sleeve that covered the outside walls of the chamber. The unconditioned stimulus (US) was two, 1.5 mA foot-shocks, each 2s in duration, and presented 1s apart immediately upon chamber entry. Videos of each session (preexposure, training, testing) were recorded using Freeze-Frame 3.0 software (Actimetrics, Wilmette IL) with freezing defined as a bout of 0.75 s or longer without a change in video pixilation.

Heroux N.A., Horgan C.J., Rosen J.B, & Stanton M.E. (2019). Cholinergic rescue of neurocognitive insult following third-trimester equivalent alcohol exposure in rats. Neurobiology of learning and memory, 163, 107030.

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