Avance ultrashield 400 mhz spectrometer

X-ray powder diffraction (XRPD) patterns were obtained using a D8 Advance (Bruker AXS, Germany), with a copper Kα radiation source (40 kV and 40 mA) with a Lynx Eye detector. The 2-theta scan range was of 5–40 degrees. Fourier-transform infrared (FTIR) spectra of all the MOFs and loaded MOFs were measured using a PerkinElmer FTIR spectrometer in the range of 650–3,800 cm⁻¹. The surface morphology imaging of the MOFs was achieved by using Zeiss Neon 40 EsB field-emission scanning electron microscopy (SEM) with SmartSEM software. Nuclear magnetic resonance (NMR) spectroscopy provides a better understanding of the loading behavior in the drug delivery system. NMR spectra were acquired on a Bruker Avance Ultrashield 400 MHz spectrometer and the NMR spectra were referenced to their solvents: deuterium oxide (D₂O, 1H, δ4.79 ppm). Also, textural properties of MOFs were characterized by N₂ adsorption–desorption isotherms, which were obtained at −196°C by Micromeritics, Tristar instrument, to determine the specific surface area, pore size, and volume.