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Mach1 t1

Manufactured by Thermo Fisher Scientific
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The MACH1-T1 is a compact and versatile laboratory instrument designed for high-throughput sample processing. It features an automated liquid handling system capable of precise pipetting and accurate volume dispensing. The MACH1-T1 is suitable for a wide range of applications in research and diagnostic laboratories.

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Mach1 (29 citations) One-Shot Mach1 T1 (7 citations) Escherichia coli Mach1 T1 cells (2 citations) One-Shot Mach1 T1 Chemically Competent cells (2 citations) One Shot Mach1 T1 Phage-Resistant Chemically Competent Escherichia coli (2 citations) One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli cells (2 citations)

2 protocols using mach1 t1

1

Bacterial Cloning Using pBABE Plasmid

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The Escherichia
coli (E. coli) bacterial strain MACH1-T1
(Invitrogen) was used as the host for cloning. The vector used was
a derivative of the retroviral pBABE-puro plasmid (http://www.addgene.org/1764, Garry Nolan) with the SfiI site deleted.
Telmer C.A., Verma R., Teng H., Andreko S., Law L, & Bruchez M.P. (2015). Rapid, Specific, No-wash, Far-red Fluorogen Activation in Subcellular Compartments by Targeted Fluorogen Activating Proteins. ACS Chemical Biology, 10(5), 1239-1246.
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2

Cloning and Construct Design for Fusion Proteins

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The Escherichia coli (E. coli) bacterial strain MACH1-T1
(Invitrogen) was used as the host for cloning. The pET21a vector was
modified to include an N-terminal 10XHis and GST followed by an HRV3C
protease cleavage site. Multiple cloning sites were introduced after
the HRV3C protease site in the order of HindIII,
NheI, BamHI, SpeI, KpnI, and XhoI
from 5′ to 3′. Fragments of FAPdL5** and
affibody ZEGFR:1907 were amplified from pPNL629 (link) and pJET1.2, respectively. In construct ZEGFR:1907, affibody was inserted into the modified pET21a vector
using HindIII and XhoI sites. FAP was inserted between
NheI and BamHI sites to make construct FAPdL5:**. In construct FAPdL5**-ZEGFR:1907, FAP was
introduced into the vector between HindIII and NheI
sites; affibody was inserted between KpnI and XhoI
sites. In constructs ZEGFR:1907-FAPdL5**, FAP
and affibody were introduced using KpnI, XhoI, HindIII, and NheI sites. For construct ZEGFR:1907-FAPdL5**-ZEGFR:1907, affibody was introduced
into ZEGFR:1907-FAPdL5** construct through HindIII and BamHI sites.
Wang Y., Telmer C.A., Schmidt B.F., Franke J.D., Ort S., Arndt-Jovin D.J, & Bruchez M.P. (2014). Fluorogen Activating Protein–Affibody Probes: Modular, No-Wash Measurement of Epidermal Growth Factor Receptors. Bioconjugate Chemistry, 26(1), 137-144.
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