Plan apochromat 20x 0.8 na air objective

Manufactured by Zeiss

The PLAN APOCHROMAT 20X/0.8 NA air objective is a high-performance objective lens designed for optical microscopy. It features an Apochromatic correction and a numerical aperture of 0.8, providing excellent image quality and resolution. The objective is suitable for use in air-based microscopy applications.

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Lab products found in correlation

Axio imager, by Zeiss (2 mentions) W plan apo 1.0 na 20x water immersion objective, by Zeiss (2 mentions) Spectra x light engine led light, by Lumencor (2 mentions) Orca flash 4.0 scmos camera, by Hamamatsu Photonics (2 mentions) Axio examiner z1, by Zeiss (2 mentions) Vectashield h 1000, by Vector Laboratories (1 mentions) Axio observer z1, by Zeiss (1 mentions)

Close spelling variants of this product

Plan-Apochromat 20x/0.8 air objective 20× Plan-Apochromat 0.8 NA air objective Plan-Apochromat 20x/0.8 NA Plan-Apochromat 20X/0.8 objective 20X/NA 0.8 plan apochromat objective Plan-Apochromat 20x NA 0.8 20× 0.8 NA air objective Plan-Apochromat 20x objective Plan-Apochromat air objective Plan-Apochromat 20x/0.8

4 protocols using plan apochromat 20x 0.8 na air objective

Quantifying GFP-LC3 puncta in HeLa cells

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HeLa/GFP-LC3 cells^{51 (link)}were imaged using a Zeiss AxioImager Z2 microscope equipped with a Photometrics
CoolSnap HQ2 camera using a Zeiss PLAN APOCHROMAT 20X/0.8 NA air objective.
GFP-LC3 puncta were quantified on images captured using the Zeiss Z2 microscope
by an observer blinded to experimental conditions.

Chiang W.C., Wei Y., Kuo Y.C., Wei S., Zhou A., Zou Z., Yehl J., Ranaghan M.J., Skepner A., Bittker J.A., Perez J.R., Posner B.A, & Levine B. (2018). High Throughput Screens to Identify Autophagy Inducers that Function by Disrupting Beclin 1/Bcl-2 Binding. ACS chemical biology, 13(8), 2247-2260.

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Fluorescent In-Situ Hybridization Imaging

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Dissected brains were coverslip mounted in Vectashield (H-1000, Vector Labs) or 80% glycerol in PBS and imaged using a compound microscope (Axioimager with EC Plan-Neofluar 10x/0.30 NA air objective or Plan-Apochromat 20x/0.8 NA air objective, Zeiss) for chromogenic ISH samples, or for double fluorescent ISH samples, a confocal microscope (LSM 780 with Plan-Apochromat 10x/0.45 NA air objective, LD LCI Plan-Apochromat 25x/0.8 NA Imm Corr objective, or LD C-Apochromat 40x/1.1 NA water objective, Zeiss). Fluorescein and cyanine were imaged in separate channels with 488 nm and 561 nm lasers, respectively. Confocal images are displayed as the maximum intensity z-projection of a stack of optical sections of approximately one airy unit (A.U.) thickness.

Lee D.A., Andreev A., Truong T.V., Chen A., Hill A.J., Oikonomou G., Pham U., Hong Y.K., Tran S., Glass L., Sapin V., Engle J., Fraser S.E, & Prober D.A. (2017). Genetic and neuronal regulation of sleep by neuropeptide VF. eLife, 6, e25727.

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Spontaneous Activity Imaging of Neurons

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Spontaneous activity imaging was performed on an upright AxioExaminer Z-1 (Zeiss) or an inverted Zeiss AxioObserver Z-1 (Zeiss), both equipped with a Spectra-X light engine LED light (Lumencor), and controlled with µManager (V1.4, open-source, Open Imaging) (Edelstein et al., 2014) . Images were acquired using a W-Plan-Apo/1.0 NA 20x water immersion objective (Zeiss) or a Plan-Apochromat/0.8 NA 20x air objective (Zeiss).
Images (2048 x 400 px 2 , pixel size: 0.325 x 0.325 μm 2 ) were collected continuously on an OrcaFlash4.0 sCMOS camera (sCMOS; Hamamatsu) at a sampling rate of 0.5 kHz, with 4x4 binning, and a 633 nm LED excitation light power of 6.5 mW/mm 2 . 6 x 10 s movies were recorded sequentially for each imaging area.

Walker A., Raliski B.K., Nguyen D.V., Zhang P., Sanders K., Karbasi K., & Miller E.W. (2020). Imaging voltage in complete neuronal networks within patterned microislands reveals preferential wiring of excitatory hippocampal neurons.

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Simultaneous Calcium Imaging in 3D

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Spontaneous activity imaging was performed on an upright AxioExaminer Z-1 (Zeiss) or an inverted Zeiss AxioObserver Z-1 (Zeiss), both equipped with a Spectra-X light engine LED light (Lumencor), and controlled with Slidebook (3i). Images were acquired using a W-Plan-Apo/1.0 NA 20x water immersion objective (Zeiss) or a Plan-Apochromat/0.8 NA 20x air objective (Zeiss). Images (2048 x 400 px 2 , pixel size: 0.325 x 0.325 μm 2 ) were collected continuously on an OrcaFlash4.0 sCMOS camera (sCMOS; Hamamatsu) at a sampling rate of 0.5 kHz, with 4x4 binning, and a 633 nm LED excitation light power of 13 mW/mm 2 .

Walker A., Raliski B.K., Karbasi K., Zhang P., Sanders K., & Miller E.W. (2020). Optical spike detection and connectivity analysis with a far-red voltage-sensitive fluorophore reveals changes to network connectivity in development and disease.

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