Pre-B cell line (70Z/3) was a gift from Brian Seed (Harvard University) (31 (link)). 4T1, NMuMG and NMuMG* subline with silencing of hnRNP E1 were described previously (32 (link)). 70Z/3 was validated by flow cytometry using B cell lineage markers. Cancer cells were authenticated by gene expression analysis, in vivo growth and histology. All the lines were monitored for pathogens as per MUSC regulations and we routinely perform mycoplasma analysis on the lines. 293FT and other cell lines were purchased from ATCC.
BALB/c and NOD–Rag-1−/− mice were purchased from The Jackson Laboratory (Bar Harbor, ME). All mouse experiments were approved by MUSC’s Institutional Animal Care and Use Committee, and the established guidelines were followed.
BALB/c and NOD–Rag-1−/− mice were purchased from The Jackson Laboratory (Bar Harbor, ME). All mouse experiments were approved by MUSC’s Institutional Animal Care and Use Committee, and the established guidelines were followed.