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Slrii

Manufactured by BD
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The SLRII is a laboratory instrument designed for cell sorting and analysis. It is a compact, modular system that offers high-performance flow cytometry capabilities. The SLRII is capable of detecting and analyzing multiple parameters of individual cells or particles within a sample.

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Lab products found in correlation

Leukocyte activation cocktail, by BD (2 mentions) Live dead fixable dead cell stain kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

BD SLRII Fortessa flow cytometer (3 citations)

2 protocols using slrii

1

Intracellular Cytokine Staining and FACS Analysis

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After blocking FcR, cells were incubated with appropriately diluted antibodies. Acquisition was performed using a SLRII (BD Biosciences, Mountain View, CA) and data analysis was conducted using FlowJo software (Tree Star Inc., Ashland, OR). For intracellular cytokines staining, cells were re-stimulated with BD Leukocyte Activation Cocktail for 4 h. FACS analysis was gated on the live cells only by using LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen Life Technologies). FACS analysis of TILs was gated on live CD45+TCRβ+(or CD3+) cells.
Chen X., Yang Y., Zhou Q., Weiss J.M., Howard O.Z., McPherson J.M., Wakefield L.M, & Oppenheim J.J. (2014). Effective Chemoimmunotherapy with Anti-TGFβ Antibody and Cyclophosphamide in a Mouse Model of Breast Cancer. PLoS ONE, 9(1), e85398.
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2

Intracellular Cytokine Flow Cytometry

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After blocking FcR, cells were incubated with appropriately diluted antibodies. Acquisition was performed using a SLRII (BD Biosciences, Mountain View, CA) and data analysis was conducted using FlowJo software (Tree Star Inc., Ashland, OR). For intracellular cytokines staining, cells were re-stimulated with BD Leukocyte Activation Cocktail for 4 h. FACS analysis was gated on the live cells only by using a LIVE/DEAD® Fixable Dead Cell Stain Kit.
Chen X., Nie Y., Xiao H., Bian Z., Scarzello A.J., Song N.Y., Trivett A.L., Yang D, & Oppenheim J.J. (2016). TNFR2 expression by CD4 effector T cells is required to induce full-fledged experimental colitis. Scientific Reports, 6, 32834.
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