Trichloroacetic acid tca 1 1 3 3 tetramethoxypropane

The standard compounds, anhydroicaritin 3-O-rhamnoside (1), β-anhydroicaritin (2), epimedins A-C (3–5), epimedoside A (6), icariin (7), and des-O-methyl-β-anhydroicaritin (8), were isolated from the extract of E. koreanum by Emeritus professor Sam Sik Kang from Seoul National University. The compounds were identified by nuclear magnetic resonance (NMR) spectroscopy and their purities were assessed by NMR and thin layer chromatography (TLC) analysis before use for this study. Copper sulfate (CuSO₄), dialysis tubing cellulose membrane, trichloroacetic acid (TCA), 1,1,3,3-Tetramethoxypropane, bovine serum albumin (BSA), and dichlorofluorescein diacetate (DCFH-DA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Coomassie Brilliant Blue R-250 was purchased from Tokyo Chemical Industry (Tokyo, Japan).

The standard compounds, artemetin (1), casticin (2), hesperidin (3), luteolin (4), vitexin (5), and vanillic acid (6), were isolated from the extract of V. rotundifolia fruits by emeritus professor Sam Sik Kang from Seoul National University. The compounds were identified by NMR and their purities were assessed by NMR and TLC before use for this study. TrichloroAcetic acid (TCA), 1,1,3,3-tetramethoxypropane, copper sulfate (CuSO₄), bovine serum albumin (BSA), and dialysis tubing cellulose membrane were purchased from Sigma Aldrich (St. Louis, MO, USA). Coomassie brilliant blue R-250 and guanidine hydrochloride were obtained from Tokyo Chemical Industry (Tokyo, Japan). 2,4-Dinitrophenylhydrazine (DNPH) was purchased from Junsei Chemical Co., Ltd (Tokyo, Japan). Acetic acid and sodium chloride were purchased from Merck (Darmsstadt, Germany).