Cox 2 antibody

All chemical reagents were obtained from Sigma (St. Louis, MO, USA). AM and TH phytochemicals were supplied from NeuMed Inc. (Seoul, Korea), dissolved in dimethyl sulfoxide (DMSO) for experiment. Dextran sulfate sodium salt (DSS; molecular weight at 36,000 to 50,000 Da) was purchased from MP Biomedicals (Morgan Irvine, CA, USA). Primary antibodies for Western blotting were purchased as follows: α-tubulin, β-actin, and NQO-1 from Santa Cruz Biotechnology (Santa Cruz, CA, USA); COX-2 antibody was from Thermo Scientific (Fremont, CA, USA), other antibodies from Cell Signaling Technology (Danvers, MA, USA). Horseradish peroxidase-conjugated anti-rat/rabbit/mouse/goat IgG was purchased Santa Cruz Biotechnology. All other materials were obtained in the highest available grade.

Rutin was dissolved in DMSO to a concentration of 50mM and stored in -20℃ as a master stock solution. Dimethyl sulfoxide (DMSO), 3-(4,5-dimethyl thizol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), 2′,7′-dichlorofluorescein diacetate (H₂DCF-DA), Hoechst 33342, Thiobarbituric Acid (TBA), Hydrogen peroxide (H₂O₂), Trichloroacetic Acid,(TCA), Malondialdehyde (MDA), Propidium Iodine (PI) and actin antibody were purchased from Sigma Chemical Co. (St. Louis, MO, USA). NuPAGE Bis-Tris Electrophoresis System (pre-cast polyacrylamide mini-gel) was purchased from Invitrogen (Carlsbad, CA, USA). COX-2 antibody was purchased from Thermo scientific (Waltham, MA, USA). PARP antibodies and horseradish peroxidase - conjugated anti-mouse or anti-rabbit IgG secondary antibodies were purchased from Cell signaling (MA, USA). Polyvinyldenefluoride (PVDF) membranes, BSA protein assay kit and Western blot chemiluminescence reagent were purchased from Amersham Biosciences (Arlington Heights, IL). Superoxide dismutase activity assay kit was purchased from biovision (Mountain View, CA). Glutathione peroxidase assay kit was purchased from Cayman Chemical (MI, USA). DNA Fragmentation Assay Kit was purchased from Clontech Laboratories (Mountain View, CA). Non-Radioactive Cytotoxicity Assay was purchased from Promega (Madison, WI, USA)