5 chloro 8 hydroxyquinoline

Tested compounds (i.e. 8HQ and derivatives) and antibiotics are comercially available. 8-Hydroxyquinoline (8HQ, 1); 5-chloro-8-Hydroxyquinoline (5-Cl-8HQ or cloxyquin, 2); 5-nitro-8-Hydroxyquinoline (5-NO₂-8HQ or nitroxoline, 3); 7-bromo-8-Hydroxyquinoline (7-Br-8HQ, 4); 5,7-diiodo-8-Hydroxyquinoline (5,7-diI-8HQ or iodoquinol, 5) and 5-chloro-7-iodo-8-hydroxy- quinoline (5-Cl-7-I-8HQ or clioquinol, 6) were obtained from Sigma-Aldrich. 5,7-Dichloro-8-Hydroxyquinoline (5,7-diCl-8HQ, 7) was purchased from Acros Organics. Spectinomycin was supplied with Bio Basic Inc. Chemical structures of the tested compounds are shown in Figure 1(Fig. 1).

All solvents were of analytical grade and used without further purification (except the solutions used for the DNA cleavage assay, vide infra). [RhCp*(μ-Cl)Cl]₂, [Ru(η⁶-p-cymene)(μ-Cl)Cl]₂, 5-chloro-8-hydroxyquinoline, D-proline, D-homoproline, aqueous formaldehyde (30% (v/v)), pic, phen, KNO₃, HNO₃, KOH, n-octanol, CD₃OD, D₂O, doxorubicin, EB, HSA (A8763, essentially globulin free), ct-DNA, DG, 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS) were Sigma-Aldrich (St. Louis, MO, USA) products and were used without further purification. FHA was purchased from GenScript (Piscataway, NJ, USA). NaH₂PO₄, Na₂HPO₄, KH₂PO₄, KCl, NaCl, diethyl-ether, n-hexane, ethanol, ethyl acetate and methanol were purchased from Molar Chemicals (Halásztelek, Hungary) or Reanal (Budapest, Hungary). [RuCym(HQCl-L-Pro)Cl]Cl and [RhCp*(HQCl-L-Pro)Cl]Cl were used in some cases due to comparative reasons, and their preparation and characterization were reported in our previous work [25 (link)].

Poly(L-lactide) (PLA; Ingeo™ Biopolymer 4032D, NatureWorks LLC—USA, Minnetonka, MN, USA; M_W = 259,000 g/mol; M_W/M_n = 1.94; as determined by size-exclusion chromatography using polystyrene standards), 5-nitro-8-hydroxyquinoline (Pharmachim, Sofia, Bulgaria), and 5-chloro-8-hydroxyquinoline (Sigma-Aldrich, St. Louis, MO, USA) were used. Dichloromethane (DCM; Merck, Darmstadt, Germany) and ethanol (abs. EtOH; Merck, Darmstadt, Germany) were of an analytical grade of purity.
Potato dextrose agar medium was obtained from Merck, Darmstadt, Germany. The disposable consumables were purchased from Orange Scientific, Braine-l’Alleud, Belgium.