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Mash1

Manufactured by Abcam
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Sourced in United Kingdom, France, United States

Mash1 is a recombinant protein that can be used as a research tool. It is a member of the MASH family of transcription factors.

Automatically generated - may contain errors

Lab products found in correlation

Ctip2, by Abcam (1 mentions) Satb2, by Abcam (1 mentions) β actin, by Merck Group (1 mentions) Valproic acid, by Merck Group (1 mentions) Nestin, by Merck Group (1 mentions) Trichostatin a, by Merck Group (1 mentions) Taq polymerase, by Takara Bio (1 mentions) Protein g agarose, by Merck Group (1 mentions) Neurod1, by Abcam (1 mentions) Phospho gsk3β antibody, by Cell Signaling Technology (1 mentions) Vglut1, by Abcam (1 mentions) Synaptophysin antibody, by Abcam (1 mentions) Histone h3, by Cell Signaling Technology (1 mentions) Ecl reagent, by Cytiva (1 mentions) Hdac1, by Cell Signaling Technology (1 mentions) Acetyl histone h3, by Cell Signaling Technology (1 mentions) Gsk3β, by Cell Signaling Technology (1 mentions) Psd95, by Abcam (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Superscript 2 reverse transcriptase, by Thermo Fisher Scientific (1 mentions)

4 protocols using mash1

1

Immunostaining of Neuronal Markers

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The following antibodies were used, including Satb2, Ctip2, Tbr1, and Mash1 (Abcam, Cambridge, CB, UK), Plzf and PHH3 (Santa Cruz, Santa Cruz, CA, USA), Pax6 (Merck Millipore, Darmstadt, Hessen, Germany), β-actin (Sigma-Aldrich, Louis, MO, USA).
Lin H.C., Ching Y.H., Huang C.C., Pao P.C., Lee Y.H., Chang W.C., Kao T.J, & Lee Y.C. (2019). Promyelocytic leukemia zinc finger is involved in the formation of deep layer cortical neurons. Journal of Biomedical Science, 26, 30.
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2

Neuronal Differentiation Protocols

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The materials used in this study's experiments are listed below along with their respective suppliers: Lithium chloride, Sodium butyrate, Trichostatin A, and Valproic acid from Sigma (St. Louis, MO); ECL™ reagents from Amersham Life Science (Arlington Heights, IL); Trizol® reagent, SuperScript™II Reverse Transcriptase, Tert siRNA [TERT-RSS313560 (rat), TERT-MSS211505-7 (mouse)] from Invitrogen (Carlsbad, CA); Protein G Agarose from Millipore (Billerica, MA); TDZD-8 (4-Benzyl-2-methyl-1,2,4,-Thiadiazolidine-3,5-dione) from Calbiochem (La Jolla, CA); Taq polymerase from Takara (Shiga, Japan).
Antibodies from the following companies: β-actin antibody from Sigma (St. Louis, MO); Tuj-1 antibody from Covance (Princeton, NJ); TERT antibody from Santa Cruz Biotechnology (Santa Cruz, CA); vGluT1, PSD-95, α-CaMKII, NeuroD1, Mash1, BRG1, Synaptophysin antibody from Abcam (Cambridgeshire, England); GAD, Nestin, GFAP, Pax6 and Ngn2GFAP antibody from Millipore (Billerica, MA); HDAC1, Histone H3, Acetyl-Histone H3, GSK3β and phospho-GSK3β antibody from Cell signaling (Boston, MA).
Kim K.C., Choi C.S., Gonzales E.L., Mabunga D.F., Lee S.H., Jeon S.J., Hwangbo R., Hong M., Ryu J.H., Han S.H., Bahn G.H, & Shin C.Y. (2017). Valproic Acid Induces Telomerase Reverse Transcriptase Expression during Cortical Development. Experimental Neurobiology, 26(5), 252-265.
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3

Western Blot Analysis of Notch Pathway Proteins

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Following protein extraction with RIPA buffer, Western blot analyses were performed using standard procedure with chemiluminescence using ECL system (Bio-Rad, Marnes-la-Coquette, France), as previously detailed [39 (link)]. Antibodies against the following proteins were used: Trim71 (goat polyclonal, 1/1000, LSBio, Clinisciences), Dll1 (sheep polyclonal, 1/1000, USBiological), Notch1 (Rabbit monoclonal, 1/1000, Cell Signaling Technology), Hes1 (rabbit monoclonal, 1/1000, Cell Signaling Technology), and Mash1 (rabbit polyclonal, 1/1000, Abcam, Paris, France).
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, chicken monoclonal, 1/1000, Millipore, Fontenay-sous-Bois, France) was used as an internal standard. Polyvinylidenedifluoride membranes were incubated for 1 h at room temperature with the corresponding horseradish peroxidase-conjugated preadsorbed secondary antibody (1/2000, Santa Cruz).
Geoffroy A., Kerek R., Pourié G., Helle D., Guéant J.L., Daval J.L, & Bossenmeyer-Pourié C. (2016). Late Maternal Folate Supplementation Rescues from Methyl Donor Deficiency-Associated Brain Defects by Restoring Let-7 and miR-34 Pathways. Molecular Neurobiology, 54(7), 5017-5033.
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4

Western Blot Analysis of Neuronal Markers

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Westerns were performed and normalized against GAPDH (Glyceraldehyde 3-phosphate dehydrogenase, 37 kd, 1:10,000, MAB374, Millipore, Billerica, MA, USA) as previously reported by our group [32 (link)]. For detection of pAMPK, NaF (50 mM) was added to all buffers, which had been demonstrated to be an effective phosphoseryl and phosphothreonyl protein phosphatase inhibitor [33 (link)]. Antibodies were obtained from Santa Cruz Biotechnology, Inc (Santa Cruz, CA, USA) unless otherwise specified: AgRP (1:500, 14 kd, sc-50299); POMC (1:500, 30 kd, sc-20148); Hes1 (1:500, 35 kd, sc-25392); Mash1 (1:500, 30 kd, sc-13222); Ngn3 (1:1000, 23 kd, ab38548, Abcam, Cambridge, MA); and AMPK (1:1000, 63 kd, sc-19128). All values were normalized to GAPDH and presented as fold change.
Desai M., Ferrini M.G., Han G., Narwani K, & Ross M.G. (2020). Maternal High Fat Diet Programs Male Mice Offspring Hyperphagia and Obesity: Mechanism of Increased Appetite Neurons via Altered Neurogenic Factors and Nutrient Sensor AMPK. Nutrients, 12(11), 3326.
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