Annexin 5 pi detection kit

Manufactured by 4A Biotech

Sourced in China

The Annexin V/PI detection kit is a laboratory tool used to identify and quantify apoptotic cells. It utilizes Annexin V, a calcium-dependent phospholipid-binding protein, and propidium iodide (PI), a nucleic acid-binding dye, to differentiate between viable, early apoptotic, and late apoptotic/necrotic cells.

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Lab products found in correlation

Rnase a, by 4A Biotech (5 mentions) Fluorescence activated cell sorting flow cytometry, by Beckman Coulter (4 mentions) Flow cytometry, by Beckman Coulter (3 mentions) Propidium iodide pi, by 4A Biotech (2 mentions) Propidium iodide, by 4A Biotech (2 mentions) Ow cytometry, by Beckman Coulter (2 mentions) Uorescence activated cell sorting ow cytometry, by Beckman Coulter (2 mentions)

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Annexin V

6 protocols using annexin 5 pi detection kit

Cell Cycle and Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

SKOV3/PTX and HeyA-8/PTX cells were seeded into six-well plates and treated with PTX for 48 h. To assess the cell cycle and apoptosis, 3 × 10⁵ treated cells were seeded into six-well plates and cultured for 48 h at 37°C. The cells for cell cycle analysis were digested using trypsin (HyClone), washed twice with phosphate-buffered saline (PBS), and fixed in 70% ethanol overnight at 4°C. The cells were centrifuged at 500 × g for 5 min, washed twice with cold PBS, and centrifuged. After treating with RNase A (0.1 mg/mL) and propidium iodide (PI, 0.05 mg/mL) purchased from 4A Biotech (Beijing, China) for 30 min at 37°C, cell cycle analysis was performed through fluorescence-activated cell sorting flow cytometry (Beckman Coulter, Palo Alto, CA, USA). For the analysis of apoptosis, cells were trypsinized followed by two PBS washing steps. The cells were stained using the annexin V/PI detection kit (4A Biotech, Beijing, China) for 5 min at room temperature. The apoptotic cells were measured using flow cytometry (Beckman Coulter). All experiments were repeated at least three times.

Zhang S., Cheng J., Quan C., Wen H., Feng Z., Hu Q., Zhu J., Huang Y, & Wu X. (2019). circCELSR1 (hsa_circ_0063809) Contributes to Paclitaxel Resistance of Ovarian Cancer Cells by Regulating FOXR2 Expression via miR-1252. Molecular Therapy. Nucleic Acids, 19, 718-730.

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Cell Cycle and Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

SKOV3/PTX and HeyA-8/PTX cells were seeded into 6-well plates and treated with PTX for 48 h. To assess the cell cycle and apoptosis, we seeded 3 × 10⁵ treated cells into 6-well plates and cultured them for 48 h at 37°C. The cells for cell-cycle analysis were digested using trypsin (Hyclone), washed twice with phosphate-buffered saline (PBS), and fixed in 70% ethanol overnight at 4°C. The cells were centrifuged at 500 × g for 5 min, washed twice with cold PBS, and centrifuged. After treatment with RNase A (0.1 mg/mL) and propidium iodide (PI, 0.05 mg/mL) purchased from 4A Biotech (Beijing, China) for 30 min at 37°C, cell-cycle analysis was performed through fluorescence-activated cell sorting flow cytometry (Beckman Coulter, Palo Alto, CA, USA). For the analysis of apoptosis, cells were trypsinized followed by two PBS washing steps. The cells were stained using the Annexin V/PI detection kit (4A Biotech, Beijing, China) for 5 min at room temperature. The apoptotic cells were measured using flow cytometry (Beckman Coulter). All experiments were repeated at least three times.

Xia B., Zhao Z., Wu Y., Wang Y., Zhao Y, & Wang J. (2020). Circular RNA circTNPO3 Regulates Paclitaxel Resistance of Ovarian Cancer Cells by miR-1299/NEK2 Signaling Pathway. Molecular Therapy. Nucleic Acids, 21, 780-791.

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Cell Cycle and Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

For analysis of the cell cycle and apoptosis, 3 × 10⁵ treated cells were seeded in 6-well plates and cultured for 48 h at 37°C. For cell cycle analysis, the cells were digested using trypsin (HyClone, Logan, UT, United States), washed twice with phosphate-buffered saline (PBS), and fixed in 70% ethanol overnight at 4°C. The cells were centrifuged at 500 ×g for 5 min, washed twice with cold PBS, and centrifuged. After the cells were treated with RNase A (0.1 mg/ml) and propidium iodide (PI, 0.05 mg/ml, 4A Biotech, Beijing, China) for 30 min at 37°C, cell cycle analysis was performed using fluorescence-activated cell sorting flow cytometry (Beckman Coulter, Palo Alto, CA, USA). For analysis of apoptosis, the cells were trypsinized followed by two PBS washes. The cells were stained using the Annexin V/PI detection kit (4A Biotech) for 5 min at 25°C. Apoptotic cells were measured using flow cytometry (Beckman Coulter). All experiments were repeated at least three times.

Jiang B., Tian M., Li G., Sadula A., Xiu D., Yuan C, & Bing Y. (2022). circEPS15 Overexpression in Hepatocellular Carcinoma Modulates Tumor Invasion and Migration. Frontiers in Genetics, 13, 804848.

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Cell Cycle and Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess the cell cycle and apoptosis, 3 × 10⁵ treated cells were seeded into 6-well plates and cultured for 48 h at 37 °C. The cells for cell cycle analysis were digested using trypsin (Hyclone), washed twice with phosphate-buffered saline (PBS), and fixed in 70% ethanol overnight at 4 °C. The cells were centrifuged at 500 g for 5 min, washed twice with cold PBS, and centrifuged. After treating with RNase A (0.1 mg/ml) and propidium iodide (PI, 0.05 mg/ml) purchased from 4A Biotech (Beijing, China) for 30 min at 37 °C, cell cycle analysis was performed through fluorescence-activated cell sorting flow cytometry (Beckman Coulter, Palo Alto, CA, USA).
For the analysis of apoptosis, cells were trypsinised followed by two PBS washing steps. The cells were stained using the Annexin V/PI detection kit (4A Biotech, Beijing, China) for 5 min at room temperature. The apoptotic cells were measured using flow cytometry (Beckman Coulter). All experiments were repeated at least three times.

Xu L., Feng X., Hao X., Wang P., Zhang Y., Zheng X., Li L., Ren S., Zhang M, & Xu M. (2019). CircSETD3 (Hsa_circ_0000567) acts as a sponge for microRNA-421 inhibiting hepatocellular carcinoma growth. Journal of Experimental & Clinical Cancer Research : CR, 38, 98.

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Cell Cycle and Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess the cell cycle and apoptosis, 3×105 treated cells were seeded into 6-well plates and cultured for 48h at 37°C. The cells for cell cycle analysis were digested using trypsin (Hyclone), washed twice with phosphate-buffered saline (PBS), and xed in 70% ethanol overnight at 4°C. The cells were centrifuged at 500g for 5min, washed twice with cold PBS, and centrifuged. After treating with RNase A (0.1mg/ml) and propidium iodide (PI, 0.05mg/ml) purchased from 4A Biotech (Beijing, China) for 30min at 37°C, cell cycle analysis was performed through uorescence-activated cell sorting ow cytometry (Beckman Coulter, Palo Alto, CA, USA). For the analysis of apoptosis, cells were trypsinised followed by two PBS washing steps. The cells were stained using the Annexin V/PI detection kit (4A Biotech, Beijing, China) for 5min at room temperature. The apoptotic cells were measured using ow cytometry (Beckman Coulter). All experiments were repeated at least three time.

Tian M., Li G., Jiang B., Sadula A., Xiu D., Yuan C., & Bing Y. (2020). circEPS15 Overexpression in Hepatocellular Carcinoma Modulates Tumor Invasion and Migration.

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Cell Cycle and Apoptosis Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess the cell cycle and apoptosis, 3 × 10 5 treated cells were seeded into 6-well plates and cultured for 48 h at 37°C. For cell cycle analysis, the cells were digested using trypsin (Hyclone, Logan, UT, USA), washed twice with phosphate-buffered saline (PBS), and xed in 70% ethanol overnight at 4°C. The cells were centrifuged at 500 ×g for 5 min, washed twice with cold PBS, and centrifuged. After treating the cells with RNase A (0.1 mg/mL) and propidium iodide (PI, 0.05 mg/mL, 4A Biotech, Beijing, China) ) for 30 min at 37°C, cell cycle analysis was performed using uorescence-activated cell sorting ow cytometry (Beckman Coulter, Palo Alto, CA, USA). To analyze apoptosis, the cells were trypsinized followed by two PBS washes. The cells were stained using the Annexin V/PI detection kit (4A Biotech) for 5 min at 25°C. Apoptotic cells were measured using ow cytometry (Beckman Coulter). All experiments were repeated at least three times.

Tian M., Li G., Jiang B., Sadula A., Xiu D., Yuan C., & Bing Y. (2021). circEPS15 Overexpression in Hepatocellular Carcinoma Modulates Tumor Invasion and Migration.

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