Anti vdac2

Manufactured by Proteintech

Sourced in United States

Anti-VDAC2 is a primary antibody that specifically recognizes the Voltage-Dependent Anion Channel 2 (VDAC2) protein. VDAC2 is a mitochondrial membrane protein that functions as a channel for the passage of small molecules and ions across the outer mitochondrial membrane. This antibody can be used in various research applications to detect and study the expression and localization of VDAC2 in biological samples.

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Lab products found in correlation

Rabbit anti hpv16 e7, by Biorbyt (1 mentions) Rabbit anti vdac1, by Proteintech (1 mentions) Anti vdac3, by Proteintech (1 mentions) Image pro plus, by Media Cybernetics (1 mentions) Cellsens dimension, by Olympus (1 mentions) Anti hsp60, by Santa Cruz Biotechnology (1 mentions) Anti atp5a, by Abcam (1 mentions) Anti myc tag, by Cell Signaling Technology (1 mentions) Anti ndufs4, by Novus Biologicals (1 mentions) Hrp conjugated goat anti rabbit igg, by Southern Biotech (1 mentions) Anti β actin, by Novus Biologicals (1 mentions) Anti mouse alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 anti rabbit, by Thermo Fisher Scientific (1 mentions) Hrp conjugated goat anti mouse igg, by Southern Biotech (1 mentions) Anti sdha, by Santa Cruz Biotechnology (1 mentions) Anti lamin b1, by Santa Cruz Biotechnology (1 mentions) Anti iqgap1, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Proteintech (1 mentions)

Close spelling variants of this product

Anti-TM

2 protocols using anti vdac2

Immunohistochemical analysis of VDAC protein expression

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IHC reactions were performed on TMA slides as described elsewhere. The TMA slides were incubated overnight in a humidified chamber at 4°C with primary antibodies: rabbit anti- VDAC1, anti-VDAC2, and anti-VDAC3 (1:300, Proteintech, USA) and rabbit anti-HPV16 E7 (1:200, Biorbyt). Antibody detection was performed with 3,3′-diaminobenzidine (DAB). CellSens Dimension (version 1.8.1, Olympus) were used to photograph the images and the staining intensity was measured with ImagePro Plus (version 6.0, Media Cybernetics).

Zhang C., Ding W., Liu Y., Hu Z., Zhu D., Wang X., Yu L., Wang L., Shen H., Zhang W., Ren C., Li K., Weng D., Deng W., Ma D, & Wang H. (2016). Proteomics-based identification of VDAC1 as a tumor promoter in cervical carcinoma. Oncotarget, 7(32), 52317-52328.

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Mitochondrial Profiling Protocol: Antibody Panel

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Unless stated otherwise, all chemicals were purchased from Sigma-Aldrich or ThermoFisher Scientific. The following antibodies were used: anti-β-actin (Novus Biologicals, Cat# NB600-501), anti-ATP5A (Abcam, clone 15H4C4, Cat# ab14748), anti-COX4 (Novus Biologicals, Cat# NB110-39115), anti-GAPDH (Protein Tech, Cat# 60004–1-Ig), anti-HSP60 (Santa Cruz, clone B-9, Cat# sc-271215 or Protein Tech, Cat# 15282–1-AP), anti-IQGAP1 (Santa Cruz, clone D-3, Cat# sc-374307 or clone C-9, Cat# sc-376021), anti-Lamin B1 (Santa Cruz, clone A-11, Cat# sc-377000), anti-Myc-Tag (Cell Signaling, clone 9B11, Cat#2276), anti-NDUFS4 (Novus Biologicals, Cat# NBP1-89026-25), anti-NDUFA4 (Santa Cruz, clone 2G7, Cat# sc-517091), anti-NDUFS1 (Santa Cruz, clone E-8, Cat# sc-271510), anti-NDUFAF2 (mimitin) (Santa Cruz, clone H-11, Cat# sc-365592), anti-SDHA (Santa Cruz, clone F-2, Cat# sc-390381), anti-UQCRC2 (Santa Cruz, clone G-10, Cat# sc-390378), anti-VDAC2 (Protein Tech, Cat# 11663-I-AP), HRP-conjugated goat anti-rabbit IgG (SouthernBiotech, Cat# 4050–05), HRP-conjugated goat anti-mouse IgG (SouthernBiotech, Cat# 1030–05), anti-rabbit-Alexa Fluor 555 (Molecular Probes, Cat# A27039), anti-rabbit-Alexa Fluor 594 (Molecular Probes, Cat# A21207), anti-mouse Alexa Fluor 488 (Molecular Probes Cat# A28175).

Papadaki V., Erpapazoglou Z., Kokkori M., Rogalska M.E., Potiri M., Birladeanu A., Tsakiri E.N., Ashktorab H., Smoot D.T., Papanikolopoulou K., Samiotaki M, & Kafasla P. (2023). IQGAP1 mediates the communication between the nucleus and the mitochondria via NDUFS4 alternative splicing. NAR Cancer, 5(3), zcad046.

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