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Magnesium chloride

Manufactured by BD
Sourced in United Kingdom

Magnesium chloride is a chemical compound with the formula MgCl2. It is a white, crystalline solid that is highly soluble in water. Magnesium chloride is commonly used in various laboratory applications as a source of magnesium ions and chloride ions.

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3 protocols using magnesium chloride

1

Optimized Chemical Reagents for Biomolecular Analysis

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Adenosine triphosphate and acetylcholine iodide were obtained from Sigma Chemical Company. Chlorpyrifos was purchased from Nantong Jinling Agricultural Chemical Co., LTD. China. Sodium barbital, potassium dihydrogen phosphate, sodium chloride, Tris, EDTA, magnesium chloride, potassium chloride, calcium chloride, ammonium molybdate, aminonaphthol sulfonic acid (ANSA) were either obtained from BDH Ltd. Poole, England or Scharlab S.L. Spain. All reagents and chemicals used were of analytical grade.
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2

Hepatic Metabolite Profiling Protocol

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Water was
obtained from Milli-Q connected to an Elix Millipore water purification
system (Millipore, USA). Acetonitrile HPLC grade, ammonium formate,
and magnesium chloride were obtained from BDH laboratory supplies
(Poole-UK). Nicotinamide adenine dinucleotide phosphate (NADPH) reduced
form were obtained from ACROS, USA. Plinabulin (NPI2358) was obtained
from Selleck Chemicals, USA. Pooled male human liver microsomes (M0567)
were purchased from Sigma-Aldrich company (West Chester, PA, USA).
The protein content of HLMs was 20 mg/mL protein in 250 mM sucrose.
HepaRG Cells Cryopreserved (HPRGC10) were purchased from ThermoFisher
company (Waltham, MA USA).
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3

Isolation and Purification of Biological Samples

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The following were purchased from the sources indicated; antihuman IgE, aprotinin, BSA, collagenase, dimethyl sulphoxide, DNase, Dowex AG-50W, Dowex AG1-X8, dithiothreitol, HSA, leupeptin, Percoll, phenyl methyl sulphonyl fluoride, ethylenediaminetetraacetic acid,, calcium chloride and magnesium chloride (BDH, Poole, UK); isoprenaline, salbutamol, (Sigma Chemical Co., Poole, UK).
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