Jurkat T cell line (clone E6.1) was obtained from ATCC and 293T cells from Cell Genesys (Foster City, CA). Jurkat cells and primary T cells were maintained in RPMI-1640 and 293T cells were maintained in Dulbecco’s modified Eagle’s medium. Media were supplemented with 10% heat-inactivated FCS, 2 mM Glutamax, 100 IU/ml penicillin, and 100 µg/ml streptomycin. Cells were grown at 37 °C in 5% CO2.
Cell viability was determined using a trypan blue exclusion method and counted using the Countess II FL automated cell counter (Invitrogen). 293F suspension cells, obtained from Thermo Fisher Scientific, were maintained in shaker flasks at 120 r.p.m., under 8% CO2 at 37 °C and propagated in their respective FreeStyle media (serum-free/defined).
Cell viability was determined using a trypan blue exclusion method and counted using the Countess II FL automated cell counter (Invitrogen). 293F suspension cells, obtained from Thermo Fisher Scientific, were maintained in shaker flasks at 120 r.p.m., under 8% CO2 at 37 °C and propagated in their respective FreeStyle media (serum-free/defined).