For the quantification of tumor volume in vivo, a subset of both female and male mice was scanned 2–7 days before euthanasia with a 7 T MRI (magnetic resonance imaging, BioSpec 70/30, 7.0 Tesla, gradient insert: BGA-12S HP, transmit volume resonator (86 mm inner diameter) and receive-only 2x2-array surface coil (all Bruker BioSpin GmbH, Ettlingen, Germany). For scanning, animals were anesthetized with 1.0–2.5 vol.% isoflurane and were placed in a supine position on the bed of the scanner. The scanning protocol comprised three orthogonal morphological T2-weighted TurboRARE (Rapid Acquisition with Relaxation Enhancement) sequences with the parameters specified in Table S4 . Tumor volume was further quantified in the axial slice faction with the software program ITK-SNAP 3.8.0 [47 (link)]. During all imaging procedures, the breathing rate and body temperature were monitored and the temperature of the animals was kept constant by a heating pad. All sequences were triggered by respiration.
B ga 12s hp
Manufactured by Bruker
Sourced in Germany
The B-GA 12S HP is a high-performance gas analyzer designed for laboratory applications. It provides accurate measurements of various gas components, including oxygen, carbon dioxide, and other specific gases. The core function of the B-GA 12S HP is to analyze and quantify the composition of gas samples in a controlled laboratory environment.
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Lab products found in correlation
5 protocols using b ga 12s hp
1
In Vivo Tumor Volume Quantification
2
Magnetic Resonance Imaging of Cerebellum
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Magnetic resonance imaging procedures were run once a week, using a dedicated phased-array quadrature head coil in a gradient/shims insert B-GA 12S HP (660 mT/m intensity and 4570 T/m/s maximum slew rate) on a 4.7 T preclinical magnet (Biospec 47/40, Bruker, Ettlingen, Germany). The first MRI was performed before the first injection, and subsequent MRI examinations were performed once a week (just before the first injection of the week, ie, 72 hours after the last injection of the previous week). An MRI examination consisted of a T1-weighted 2D FLASH (repetition time/echo time, 50/1.782 milliseconds; 48 averages; in-plane resolution, 164 × 164 μm2; slice thickness, 700 μm; acquisition time, 6 minutes 36 seconds; targeted only on the cerebellum [11 slices], and T1 mapping on the slice displaying the DCN), using a FAIR-RARE (flow-sensitive alternating inversion recovery–rapid acquisition with relaxation enhancement) sequence (repetition time/effective echo time, 36.9/2079.9 milliseconds; 4 averages; in-plane resolution, 164 × 164 μm2; slice thickness, 700 μm; acquisition time, 11 minutes 5 seconds), with 8 inversion times (0, 100, 200, 400, 600, 800, 1200, 2000 milliseconds).
3
Longitudinal Cerebellum MRI in Rats
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T1-weighted MRI was performed under general anesthesia (3%–3.5% isoflurane) at weeks 3, 4, 5, 6, and 7 (ie, after 4, 8, 12, 16, and 20 administrations, respectively) during the administration period (Fig. 1 ). During the treatment-free period, MRI was performed on weeks 9, 12, and 15. A preinjection MRI was performed only in adult rats. For logistic reasons, MRI was performed at random on 4 rats per sex and per group for the juvenile animals (groups 1, 2, and 3). Magnetic resonance imaging was performed with a dedicated phased-array quadrature head coil in a gradient/shims insert B-GA 12S HP (660 mT/m intensity and 4570 T/m per second maximum slew rate) on a 4.7 T preclinical magnet (Biospec 47/40; Bruker, Ettlingen, Germany). A T1-weighted 2D FLASH sequence was used: repetition time/echo time, 50/1.78 milliseconds; 48 averages; in-plane resolution, 164 × 164 μm2; slice thickness, 700 μm; acquisition time, 6 minutes 36 seconds. The scan range of the MRI sequence covered only the cerebellum (11 slices).
4
Longitudinal MRI Characterization of Mice
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The MRI experiments were performed on a 9.4 T Biospec 94/20 USR (Bruker BioSpin GmbH, Germany) small animal system equipped with a 675 mT/m gradient system B-GA12S HP (Bruker BioSpin GmbH, Germany). For signal acquisition a quadrature transmit/receive coil with an inner diameter of 35 mm (M2M imaging corporation, USA) was used. Imaging experiments were performed every other month starting at an age of two months until twelve months. Relaxometry data was acquired according to this scheme starting at the age of four months. The mice were anesthetized by isoflurane inhalation, initiated with 5% and then sustained with 1–3% isoflurane in 100% oxygen. Anesthetized animals were placed in supine position and covered by a warming cover, which was connected to a heating circuit. A small animal physiological monitoring system (SA Instruments, USA) was used to monitor respiration rate by a pressure sensitive pad and body temperature by a rectal thermometer. Prior to imaging body weight of each individual animal was recorded.
5
Multi-modal MRI protocol for rat brain
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All MR images were acquired with a Bruker Biospec 94/30 small animal MRI system operating at 9.4 T (400 MHz, H-1), with an Avance III HD console, BGA-12SHP imaging gradients, a 72 mm (inner diameter) volume transmit coil and a rat brain surface quadrature receive coil using the imaging protocol as described in detail in Seewoo et al. (2018; (link)2019) . Briefly, the acquisition protocol included the following sequences: 1) multi-slice 2D RARE (rapid acquisition with relaxation enhancement) sequence for three T2-weighted anatomical scans (TR=2500 ms, TE=33 ms, matrix=280x280, pixel size=0.1x0.1 mm 2 , 21 coronal and axial slices, 20 sagittal slices, thickness=1 mm); 2) single-shot gradient echo EPI (TR=1500 ms, TE=11 ms, matrix=94x70, pixel size=0.3x0.3 mm 2 , 21 coronal slices, thickness=1 mm, flip angle=90°, 300 volumes, automatic ghost correction order = 1, receiver bandwidth = 300 kHz) for resting-state; and 3) pointresolved spectroscopy (PRESS) sequence with one 90° and two 180° pulses and water suppression for 1 H-MRS (TE=16 ms, TR=2500 ms) with 64 averages with a 3.5x2×6 mm 3 voxel placed over the left sensorimotor cortex (Fig. 2). 1 H-MRS data was acquired from the left sensorimotor cortex only in order to facilitate comparisons with human depression studies, which mostly examine neurometabolite changes in the left hemisphere (Moriguchi et al., 2019) (link).
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