Paraffin tumor sections were subjected to double immunofluorescence staining by sequential method.14 (link) Primary antibodies used were endomucin (1:100; Santa Cruz Biotechnology), α-smooth muscle actin (αSMA; 1:100; Thermo Scientific), Notch1 (1:50; R&D Systems), Dll4 (1:50; R&D Systems), and Jagged1 (1:50; R&D Systems). AlexaFluor 488 and AlexaFluor 568 (Thermo Scientific) conjugates were used for fluorescence staining. For quantification, an average of 8 to 10 images, taken under 20x magnification, and 3 tumors per group were analyzed. Area (%) was determined using Fiji24 (link) using an arbitrary threshold applied to all images.
Notch1
Manufactured by R&D Systems
Notch1 is a cell surface protein that functions as a receptor for the Notch signaling pathway. It plays a crucial role in cell-cell communication and is involved in various developmental processes.
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Lab products found in correlation
3 protocols using notch1
1
Immunofluorescence Analysis of Tumor Markers
2
Immunofluorescence Analysis of Tumor Markers
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Paraffin tumor sections were subjected to double immunofluorescence staining by sequential method.14 (link) Primary antibodies used were endomucin (1:100; Santa Cruz Biotechnology), α-smooth muscle actin (αSMA; 1:100; Thermo Scientific), Notch1 (1:50; R&D Systems), Dll4 (1:50; R&D Systems), and Jagged1 (1:50; R&D Systems). AlexaFluor 488 and AlexaFluor 568 (Thermo Scientific) conjugates were used for fluorescence staining. For quantification, an average of 8 to 10 images, taken under 20x magnification, and 3 tumors per group were analyzed. Area (%) was determined using Fiji24 (link) using an arbitrary threshold applied to all images.
3
NSCLC and SM Protein ELISA
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PTK2B (ORIGENE) and NOTCH1 (R&D systems), as the representatives of NSCLC and SM groups, were selected for ELISA, respectively (see Supplementary information).
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