Fuc, Glc, Gal, 2-AA (anthranilic acid), and sodium cyanoborohydride were purchased from Nacalai Tesque (Kyoto, Japan). GlcNAc and Lac were purchased from Wako Pure Chemical Industries (Osaka, Japan). LNnT and LNFP I were purchased from Dextra Laboratory (Reading, UK). 2′-FL, 3-FL, LDFT, and LNT were obtained from IsoSep (Tullinge, Sweden), or provided as gifts from Glycom A/S (Hørsholm, Denmark). LNFP II, LNFP III, and LNDFH I were purchased from Carbosynth (Berkshire, UK), and isomaltoheptaose was purchased from Seikagaku Kogyo (Tokyo, Japan). LNB was synthesized as described previously [64 (link)]. All reagents employed in this study were of analytical grade.
Sodium cyanoborohydride
Manufactured by Nacalai Tesque
Sourced in Japan
Sodium cyanoborohydride is a reducing agent used in organic synthesis. It is commonly utilized in the reductive amination of aldehydes and ketones to form secondary amines.
Automatically generated - may contain errors
Lab products found in correlation
Lnfp 2, by Biosynth (3 mentions)
Glcnac, by Fujifilm (1 mentions)
Lndfh 1, by Biosynth (1 mentions)
Isomaltopentaose, by Seikagaku (1 mentions)
Lnfp 1, by Dextra Laboratories (1 mentions)
6 sialyllactose 6 sl, by Biosynth (1 mentions)
3 sialyllactose 3 sl, by Biosynth (1 mentions)
Dextran, by Nacalai Tesque (1 mentions)
Acetone, by Fujifilm (1 mentions)
Glycol chitosan, by Fujifilm (1 mentions)
Albumin from bovine serum, by Fujifilm (1 mentions)
Diethylene glycol, by Fujifilm (1 mentions)
4 protocols using sodium cyanoborohydride
1
Synthesis and Characterization of Glycans
2
Glycan Standards Synthesis and Acquisition
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2′-FL, 3-FL, LNnT, LNFP I, and LNDFH II were purchased from Dextra Laboratory (Reading, UK). LDFT and LNT were obtained from IsoSep (Tullinge, Sweden), or as gifts from Glycom A/S (Denmark). LNFP II and LDFH I were purchased from Carbosynth (Berkshire, UK). DFJ was obtained from Sigma-Aldrich (MO, USA). Isomaltopentaose was from Seikagaku Kogyo (Tokyo, Japan), while LNB was synthesized as previously described48 (link). 2-AA and sodium cyanoborohydride were obtained from Nacalai tesque (Kyoto, Japan). All other reagents were of analytical grade.
3
Purification and Characterization of HMOs
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2′-FL, 3-FL, LDFT, and LNT were from Sigma-Aldrich (St. Louis, MO, USA) or as gifts from Glycom A/S (Hørsholm, Denmark). LNnT and LNFP I were from Dextra Laboratories (Reading, UK). LNFP II, 3′-sialyllactose (3′-SL), and 6′-sialyllactose (6′-SL) were from Carbosynth (Berkshire, UK). Melibiose and GOSs were from FUJIFILM Wako Pure Chemical (Osaka, Japan). Maltoheptaose was from Toronto Research Chemicals (Toronto, ON, Canada). Purity of the purchased or gifted sugars is >95% according to material safety data sheets. 2-Aminoanthranilic acid and sodium cyanoborohydride were from Nacalai Tesque (Kyoto, Japan). HMOs were purified from pooled breast milk as described previously (11 (link)). The HMOs analyzed in this study accounted for >80% of the purified HMO mixture by weight, and 2% (w/w) Lac was present as impurity based on high-performance liquid chromatography (HPLC) analysis (see below). All other chemicals were of analytical grade.
4
Glycol Chitosan-Based Fluorescent Conjugates
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Glycol chitosan (GC, D DP200, degree of acetylation = 9.13 ± 0.89% as determined by 1 H-NMR), diethylene glycol, albumin from bovine serum (BSA) and acetone were purchased from Wako Pure Chemical Industries, Ltd (Osaka, Japan). Dextran (Dex, Mn=50,000-70,000), sodium periodate, sodium cyanoborohydride and fluorescein isothiocyanate isomer Ⅰ (FITC) were purchased from Nacalai tesque (Kyoto, Japan). Tertbutyl carbazate was purchased from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan).
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