Apatinib

Manufactured by Merck Group

Sourced in United States

Apatinib is a small-molecule tyrosine kinase inhibitor. It functions by inhibiting the vascular endothelial growth factor receptor (VEGFR).

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Lab products found in correlation

Apatinib, by Nikon (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) N acetyl l cysteine nac, by Merck Group (1 mentions) Inverted microscope, by Nikon (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Proteinase inhibitor, by Beyotime (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Cordycepin, by Merck Group (1 mentions) Bicinchoninic acid bca protein assay kit, by Beyotime (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Keygen Biotech (1 mentions)

4 protocols using apatinib

Apatinib Modulates NSCLC Cell Behavior

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Human NSCLC cell lines A549 and H1299, monocyte cell line THP-1 and Jurkat T cells were obtained from the Cell Bank of Chinese Academy of Sciences (Shanghai, China). The above cells were cultured in RPMI-1640 (Gibco, USA) with 10% fetal bovine serum (FBS) and 1% antibiotics.
To analyze the effect of apatinib on NSCLC, A549 and H1299 cells were treated with different concentrations of apatinib (0, 2, 5, 10 μM), using 0.1% dimethyl sulfoxide (DMSO) as the solvent control and cell morphology was observed under an inverted microscope ((Nikon, Tokyo, Japan). For combination treatment, cells were exposed to apatinib with or without 5 mM of N-acetyl-L-cysteine (NAC, Sigma-Aldrich, St. Louis, USA) or 20 ng/mL of IL-6 (Peprotech, Rocky Hill, NJ, USA). Apatinb were obtained from Hengrui Medicine Co. Ltd. (Jiangsu, China) and dissolved in DMSO (Sigma-Aldrich).

Xie C., Zhou X., Liang C., Li X., Ge M., Chen Y., Yin J., Zhu J, & Zhong C. (2021). Apatinib triggers autophagic and apoptotic cell death via VEGFR2/STAT3/PD-L1 and ROS/Nrf2/p62 signaling in lung cancer. Journal of Experimental & Clinical Cancer Research : CR, 40, 266.

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Preparation of Cordycepin and Apatinib Solutions

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Cordycepin and apatinib were obtained from Sigma (St. Louis, MO, United States). Cordycepin and apatinib dissolved in physiological saline at a concentration of 20 mM were kept in a refrigerator at −20°C for later use. The RPMI1640 medium and fetal bovine serum (FBS) were from Invitrogen (Carlsbad, CA, United States). The RIPA lysis buffer, proteinase inhibitors and bicinchoninic acid (BCA) protein assay kit were purchased from Beyotime (Nanjing, China).

Liao X., Tao L., Guo W., Wu Z.X., Du H., Wang J., Zhang J., Chen H., Chen Z.S., Lin L, & Sun L. (2020). Combination of Cordycepin and Apatinib Synergistically Inhibits NSCLC Cells by Down-Regulating VEGF/PI3K/Akt Signaling Pathway. Frontiers in Oncology, 10, 1732.

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Synergistic Anti-Cancer Regimen

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apatinib, 5‐FU, TAX and DDP were used in the present study. In vitro studies, the stock solution containing apatinib (Hengrui), 5‐FU (Sigma), TAX (Sigma) and DDP (Sigma) were prepared in dimethyl sulfoxide (DMSO). In vivo assays, apatinib was dissolved in DMSO and diluted in physiological saline. 5‐FU, TAX and DDP injection were diluted in physiological saline.

Chi Y., Wang F., Zhang Y., Shan Z., Tao W., Lian Y., Xin D., Fan Q, & Sun Y. (2022). Apatinib inhibits tumour progression and promotes antitumour efficacy of cytotoxic drugs in oesophageal squamous cell carcinoma. Journal of Cellular and Molecular Medicine, 26(7), 1905-1917.

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Isolating Breast Cancer-Derived Conditioned Medium

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Human umbilical vein endothelial cells (HUVECs) and MDA-MB-231 cells were supplied by Capital Medical University (Beijing, China). MDA-MB-231 cells (base-like) were classified as ER-, PR-, and HER2- [34] [35] [36] [37] . MDA-MB-231 cells were cultured in Dulbecco's modified Eagle medium (DMEM, Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin/streptomycin (KeyGen, Nanjing, China) and maintained in a 5% CO 2 atmosphere at a constant temperature of 37°C
. Jiangsu Hengrui Medicine Co., Ltd. (Jiangsu, China) supplied apatinib free of cost, whereas DHA was ordered from Sigma (St. Louis, MO, USA; D2534). Dimethyl sulfoxide was used to dissolve apatinib, while 100% ethyl alcohol (<0.1%) was utilized to prepare a solution of DHA. Both solutions were freshly prepared for each test.
Preparation of MDA-MB-231-conditioned medium (CM)
Isolation of MDA-MB-231-CM was performed as described previously [38, 39] . Cultures of MDA-MB -231 cells were prepared in 100-mm 2 plates until they reached 70% confluence, and then they were cultured in 0-1% FBS for 24-48 h. The medium was extracted using 0.22-μm filter film and stored at -80°C.

Ma Y., Yu J., Li Q., Su Q, & Cao B. (2020). Addition of docosahexaenoic acid synergistically enhances the efficacy of apatinib for triple-negative breast cancer therapy. Bioscience, biotechnology, and biochemistry, 84(4).

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