Nt157

Manufactured by Selleck Chemicals

Sourced in United States

NT157 is a chemical compound used in laboratory applications. It serves as a core functional component for various experimental procedures. The product's technical specifications and intended uses are not included in this factual description.

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Lab products found in correlation

Hrp conjugated goat anti mouse igg h l, by Proteintech (1 mentions) Hrp conjugated goat anti rabbit igg h l, by Proteintech (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Plx4720, by Selleck Chemicals (1 mentions) Lamin b, by Proteintech (1 mentions) Ribonuclease rnase a, by Merck Group (1 mentions) Propidium iodide, by Merck Group (1 mentions) Ab40777, by Abcam (1 mentions) Ab184787, by Abcam (1 mentions) Mifepristone ru486, by Merck Group (1 mentions) Medroxyprogesterone acetate mpa, by Merck Group (1 mentions)

3 protocols using nt157

Antibody-based Protein Detection Protocol

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Antibodies specific to the following proteins were used in this study: JAB1 (Santa Cruz, USA); Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204), p44/42 MAPK (Erk1/2), Phospho-STAT3 (Tyr705), and STAT3 from Cell Signaling Technology (Danvers, MA, USA); Lamin B, horseradish peroxidase (HRP)-conjugated beta Actin, HRP-conjugated goat anti-rabbit IgG (H + L), and HRP-conjugated goat anti-mouse IgG (H+L) were from ProteinTech; NT157 and PLX4720 were from Selleck; IL-6 was from PeproTech. The detail of dilutions and catalog number are included in supplementary materials and methods. NT157 was diluted in 20% 2-hydroxypropyl-β-cyclodextrin (2-HP-β-CD) (Selleck) for in vivo administration (100 mg/kg, intraperitoneal injection). NT157 was diluted in dimethyl sulfoxide (DMSO) for in vitro use at a concentration of 2 μM or as shown in details in the figures.

Yu S., Wang Y., Lv K., Hou J., Li W., Wang X., Guo H, & Wang W. (2021). NT157 Inhibits HCC Migration via Downregulating the STAT3/Jab1 Signaling Pathway. Technology in Cancer Research & Treatment, 20, 15330338211027916.

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Immunohistochemistry and Cell Signaling Assays

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The IRS-1 antibody for immunohistochemistry analysis was obtained from Abcam (#ab40777; Abcam, Boston, MA, USA). Similarly, the caspase 3 antibody was obtained from Abcam (#ab184787). Cell signaling (Danvers, MA, USA) provided antibodies for caspase 9 (#9502S); hexokinase (#2867S), STAT3 (#4904S), phospho STAT 3, (#9131S), cleaved PARP (#5625S), IRS-1 for western blotting (#2382S), phospho p38 MAP Kinase (#9215S), p38 MAP Kinase (#9212S), phospho AKT (#3787S), AKT (#9272S), phospho Erk1/2(#4376S), Erk1/2 (#9102S) and IGF-1R (#3027S). Phospho IGF-1R antibody was obtained from Sigma Aldrich (SAB4300652; Millipore Sigma, Burlington, MA, USA). The Actin antibody was obtained from Santa Cruz Biotechnology (#sc-47778; Santa Cruz, Dallas, TX, USA). NT157 was obtained from Selleckchem (#NM-4126; Houston, TX, USA).
Cell migration assay plates were obtained from Corning (#354578; Corning, Glendale, AZ, USA) and the staining kit was obtained from Siemens (#B4132-1A; Siemens Health care Diagnostic Inc., Newark, DE, USA). Methylthiazole tetrazolium (MTT) reagent was obtained from EMD Millipore Corp (#475989; United Kingdom). Propidium iodide was procured from Sigma Aldrich (#81845). Ribonuclease A (RNase) was obtained from Sigma-Aldrich (#R4642).

Chattopadhyay C., Bhattacharya R., Roszik J., Khan F.S., Wells G.A., Villanueva H., Qin Y., Bhattacharya R., Patel S.P, & Grimm E.A. (2022). Targeting IRS-1/2 in Uveal Melanoma Inhibits In Vitro Cell Growth, Survival and Migration, and In Vivo Tumor Growth. Cancers, 14(24), 6247.

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Pharmacological Evaluation of MPA, RU486, and NT157

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Medroxyprogesterone acetate (MPA, Cat#M1629), and mifepristone (RU486, Cat#M8046) were obtained from Sigma-Aldrich, St. Louis, MO. NT157 (Cat#S8228) was obtained from SelleckChem (Houston, USA).

Omar I.S., Abd Jamil A.H., Mat Adenan N.A, & Chung I. (2022). MPA alters metabolic phenotype of endometrial cancer-associated fibroblasts from obese women via IRS2 signaling. PLoS ONE, 17(7), e0270830.

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