Scc13

Four cSCC cell lines (A431, HSC-5, SCC13, and SCL-1) and a human keratinocyte cell line (HaCaT) were all purchased from the Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China). The cells were cultured using Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), and maintained in a 5% CO₂ atmosphere at 37°C. The pcDNA3.1-PICSAR, pcDNA3.1, miR-573 mimic, mimic negative control (NC) were purchased from GenePharma (Shanghai, China). The above vectors were transfected into cSCC cells using Lipofectamine 3000 transfection reagent (Invitrogen, CA, USA) according to the manufacturer’s protocols. Cells were collected after transfection for 48 hours and used for the following analyses.

HaCaT (one human keratinocyte cell line) and A431, HSC-5, SCC13, and SCL‐1 (four CSCC cell lines) were bought from Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China). Then the cells were cultured in DMEM (Gibco, Grand Island, NY) containing 10% FBS (Beyotime, Nantong, China) as well as 100 μg/mL streptomycin and 100 IU/mL penicillin (Invitrogen, USA) in a cell incubator with 5% CO₂ at 37 °C. HOTAIR overexpression (HOTAIR OE) plasmids, HOTAIR siRNAs, miR-326 mimics and miR-326 inhibitors were synthesized by GenePharma (Shanghai, China). On the basis of manufacturer’s protocol, Lipofectamine 2000 (Invitrogen, CA, USA) was applied to transfect cells.