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Glucose mannose fructose detection kit

Manufactured by Megazyme
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The Glucose/Mannose/Fructose detection kit is a laboratory equipment product designed to quantify the levels of glucose, mannose, and fructose in various samples. It provides a reliable and efficient method for the detection and measurement of these important monosaccharides.

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Lab products found in correlation

Cary 300 spectrophotometer, by Agilent Technologies (2 mentions) Linked galactose dehydrogenase galactose mutarotase assay, by Megazyme (2 mentions)

Close spelling variants of this product

Mannose

3 protocols using glucose mannose fructose detection kit

1

Quantifying Glycosidic Bond Cleavage

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A linked galactose dehydrogenase/galactose mutarotase assay (Megazyme) was used to quantify the release of galactose or arabinose from xyloglucan or XyGOs. The release of galactose or arabinose led to the stoichiometric reduction of NAD+ to NADH, giving an increase in A340 (ε 6230 M−1 cm−1 at pH 7.0),56 (link) which was read continuously using a Cary 300 spectrophotometer.
A second linked assay (Glucose/Mannose/Fructose detection kit, Megazyme) was used to quantify the release of glucose monosaccharides. The protocol provided in the manufacturer’s instructions was modified for use as a continuous assay. The release of a glucose monosaccharide corresponds stoichiometrically with the reduction of a molecule of NADP+ to NADPH, which leads to an increase in A340 (ε 6220 M−1 cm−1),56 (link) observed continuously using a Cary 300 spectrophotometer. Reactions were carried out in the triethylamine buffer (pH 7.6) provided with the assay kit.
Larsbrink J., Rogers T.E., Hemsworth G.R., McKee L.S., Tauzin A.S., Spadiut O., Klinter S., Pudlo N.A., Urs K., Koropatkin N.M., Creagh A.L., Haynes C.A., Kelly A.G., Cederholm S.N., Davies G.J., Martens E.C, & Brumer H. (2014). A discrete genetic locus confers xyloglucan metabolism in select human gut Bacteroidetes. Nature, 506(7489), 498-502.
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2

Quantification of Monosaccharides by HPAEC-PAD

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Commercial sugars were detected by high-pressure anion-exchange chromatography (HPAEC) with pulsed amperometric detection and were separated on a Carbopac PA1 guard and analytical column. Isocratic elution with 20 mM sodium hydroxide for 30 min and then with a 60% linear gradient of sodium acetate in 100 mM sodium hydroxide over 60 min was used to separate the sugars. Sugars were detected using the carbohydrate standard quad waveform for electrochemical detection at a gold working electrode with an Ag/AgCl pH reference electrode. The glucose/mannose/fructose detection kit from Megazyme was also utilized to detect the relative amounts of glucose and fructose. This is a linked assay kit, with the detection of the appropriate monosaccharide linked 1:1 to the detection of NADH at A340 nm using an extinction coefficient of 6230 M−1 cm−1.
Urresti S., Cartmell A., Liu F., Walton P.H, & Davies G.J. (2018). Structural studies of the unusual metal-ion site of the GH124 endoglucanase from Ruminiclostridium thermocellum. Acta Crystallographica. Section F, Structural Biology Communications, 74(Pt 8), 496-505.
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3

Quantifying Glycosidic Bond Cleavage

Check if the same lab product or an alternative is used in the 5 most similar protocols
A linked galactose dehydrogenase/galactose mutarotase assay (Megazyme) was used to quantify the release of galactose or arabinose from xyloglucan or XyGOs. The release of galactose or arabinose led to the stoichiometric reduction of NAD+ to NADH, giving an increase in A340 (ε 6230 M−1 cm−1 at pH 7.0),56 (link) which was read continuously using a Cary 300 spectrophotometer.
A second linked assay (Glucose/Mannose/Fructose detection kit, Megazyme) was used to quantify the release of glucose monosaccharides. The protocol provided in the manufacturer’s instructions was modified for use as a continuous assay. The release of a glucose monosaccharide corresponds stoichiometrically with the reduction of a molecule of NADP+ to NADPH, which leads to an increase in A340 (ε 6220 M−1 cm−1),56 (link) observed continuously using a Cary 300 spectrophotometer. Reactions were carried out in the triethylamine buffer (pH 7.6) provided with the assay kit.
Larsbrink J., Rogers T.E., Hemsworth G.R., McKee L.S., Tauzin A.S., Spadiut O., Klinter S., Pudlo N.A., Urs K., Koropatkin N.M., Creagh A.L., Haynes C.A., Kelly A.G., Cederholm S.N., Davies G.J., Martens E.C, & Brumer H. (2014). A discrete genetic locus confers xyloglucan metabolism in select human gut Bacteroidetes. Nature, 506(7489), 498-502.
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