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2 protocols using 14 15 ee5ze

1

Calcium Signaling in Cell Lines

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14,15-EET and 14,15-EE5ZE were from Cayman (Ann Arbor, MI). Quest Fluo-8 NW Calcium Assay Kit was from AAT Bioquest (AAT Bioquest Inc., Sunnyvale, CA, USA). Ca2+ free medium, capsaicin, capsazepine (CPZ), SB366791, HC030031 (HC), 1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid/acetoxymethyl ester (BAPTA/AM), ethylene glycol tetraacetic acid (EGTA), Griess's reagent, and Drabkin's reagent kit 525 were from Sigma-Aldrich (St. Louis, MO, USA). TurboFect was from Fermentas (Glen Burnie, MD, USA). Matrigel was from BD Biosciences (San Jose, CA, USA).
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2

Factors Regulating Retinal Arteriole Dilation

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To identify the factors released from NO-stimulated glial cells, we examined the effects of intravitreal injection of 14,15-EE-5(Z)-E (an analog of EET, 0.7 nmol; Cayman Chemical), 17-octadecynoic acid (an inhibitor of ω-hydroxylase, which mediates conversion of arachidonic acid to 20-HETE, 1.4 nmol; Cayman Chemical), and indomethacin (10 nmol) on intravitreal injection of NOR3 (5 nmol)-induced dilation of retinal arterioles.
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