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2 protocols using astaxanthin

1

Comprehensive Analytical Protocols for Natural Antioxidants

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All chemicals were used without further treatment. Acetonitrile, methanol, and water, when used for liquid chromatography, were purchased from Merck (Germany) as ultra-performance liquid chromatography-grade. Anhydrous sodium sulfate, sodium carbonate, formic acid, ethanol, ethyl acetate, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (≥ 98%; ABTS), Folin–Ciocalteu reagent (2M), and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (97%; Trolox®) were purchased from Merck (Germany). Gallic acid was purchased from Carbosynth (UK), astaxanthin, and lutein (90%) from Acros Organics (Thermo Fisher, Germany), and β-carotene (99%) from Alfa Aesar (Thermo Fisher, Germany). The silylation reagent (SILYL-991) was purchased from Macherey-Nagel (Germany).
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2

Carotenoid Extraction from Haematococcus pluvialis

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Freeze-dried Haematococcus pluvialis, were kindly provided by Microphyt (Baillargues, France) and stored at 4 °C until use. HPLC-grade solvents including methyl tert-butyl ether (MTBE), methanol, acetone, and ethanol were purchased from VWR (Leuven, Belgium). Sea sand (0.25-0.30 mm particle diameter) was acquired from Panreac (Castellar del Vallés, Spain). Butylated hydroxytoluene (BHT), canthaxanthin and βcarotene (from Anacystis nidulans algae) were obtained from Sigma-Aldrich (St Louis, MO, USA). Lutein (from Echinacea purpurea) was purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China). Astaxanthin was purchased from Acros Organics (Geel, Belgium), whereas zeaxanthin was acquired from Carbosynth Limited (Berkshire, UK). Ultrapure water used was obtained from a Milli-Q system (Millipore, Billerica, MA, USA). For supercritical antisolvent fractionation experiments, carbon dioxide (99% purity) was supplied by Carburos Metálicos (Barcelona, Spain).
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