Glut1 and glut4

Manufactured by Abcam

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GLUT1 and GLUT4 are membrane proteins that facilitate the transport of glucose across the cell membrane. GLUT1 is involved in the basal uptake of glucose, while GLUT4 is primarily responsible for insulin-stimulated glucose uptake in tissues like skeletal muscle and adipose tissue.

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Lab products found in correlation

Bradford reagent, by Merck Group (1 mentions) Gapdh, by Merck Group (1 mentions) Glutamine synthetase, by Abcam (1 mentions) Hexokinase 1, by Abcam (1 mentions) Protease and phosphatase inhibitor cocktail, by Merck Group (1 mentions) Glutaminase, by Abcam (1 mentions) Pyruvate carboxylase, by Santa Cruz Biotechnology (1 mentions) Histone h3, by Santa Cruz Biotechnology (1 mentions) Chemidoc touch imaging system, by Bio-Rad (1 mentions) Hdac3, by Cell Signaling Technology (1 mentions) P ampkα, by Cell Signaling Technology (1 mentions) Pai 1, by BD (1 mentions) Acetyl histone h3, by Merck Group (1 mentions) Gapdh, by Bio-Rad (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Tnf α, by Abcam (1 mentions) β actin, by Bio-Rad (1 mentions) Acetyl histone h3 lys9 lys14, by Cell Signaling Technology (1 mentions) 4 hydroxy 2 nonenal 4 hne, by Alpha Diagnostic (1 mentions) Lamin b1, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

GLUT1 (137 citations) GLUT4 (33 citations)

2 protocols using glut1 and glut4

Metabolic Profiling of Heart Tissue

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Snap-frozen heart samples were homogenized and lysed in a buffer containing 25 mM Tris·HCl, 150 mM NaCl, 2 mM EGTA, 5 mM EDTA, 0.5% NP-40, and a protease and phosphatase inhibitor cocktail (Sigma-Aldrich). Protein concentration was determined using the Bradford reagent (Sigma-Aldrich). Tissue homogenates were separated by SDS-PAGE and transferred to nitrocellulose membranes followed by Western blot analysis. Antibodies used were as follows: glucose transporters 1 and 4 (GLUT1 and GLUT4; Abcam), LDHA (Proteintech), hexokinase 1 (Abcam), PDK4 (Abcam), pyruvate dehydrogenase E1α (PDH-E1α; Abcam), phospho-PDH-E1α (S293, p-PDH-E1α; Abcam), pyruvate carboxylase (Santa Cruz), glutamine synthetase (Abcam), glutaminase (Abcam), O-linked β-N-acetylglucosamine (GlcNac RL2; Abcam), glutamine fructose amidotransferase-2 (GFAT2; Abcam), and GAPDH (Sigma-Aldrich).

Schnelle M., Chong M., Zoccarato A., Elkenani M., Sawyer G.J., Hasenfuss G., Ludwig C, & Shah A.M. (2020). In vivo [U-13C]glucose labeling to assess heart metabolism in murine models of pressure and volume overload. American Journal of Physiology - Heart and Circulatory Physiology, 319(2), H422-H431.

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Western Blot Analysis of Cellular Proteins

Cited 1 time

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Western blot was carried out as we detailed previously (23 (link)). Primary antibodies used for this assay include atrial natriuretic peptide (ANP), connective tissue growth factor (CTGF), GAPDH, Lamin B1, DUSP5, Histone H3 and β-actin (Santa Cruz Biotechnology, Dallas, TX, USA), 3-nitrotyrosine (3-NT), acetyl-Histone H3 (Millipore, Billerica, CA), 4-hydroxy-2-nonenal (4-HNE, Alpha Diagnostic International, San Antonio, TX, USA), phosphorylated c-Jun N-terminal protein kinase (p-JNK), tumor necrosis factor alpha (TNF-α), glucose transporter type 1 and 4 (GLUT1 and GLUT4) (Abcam, Cambridge, MA, USA), p-ERK1/2, ERK1/2, JNK, p-p38, p38, insulin receptor substrate 1 (IRS1), p-Akt, Akt, 5' AMP-activated protein kinase alpha (AMPKα), p-AMPKα, HDAC3 and acetyl-Histone H3 (Lys9/Lys14) (Cell Signaling Technology, Danvers, MA, USA), and plasminogen activator inhibitor-1 (PAI-1, BD Bioscience, San Jose, CA, USA). The protein blot on the nitrocellulose membrane was captured by the ChemiDoc Touch Imaging System (Bio-Rad, Hercules, CA, USA) and was normalized to that of GAPDH or β-actin.

Xu Z., Tong Q., Zhang Z., Wang S., Zheng Y., Liu Q., Qian L., Chen S.Y., Sun J, & Cai L. (2017). Inhibition of HDAC3 prevents diabetic cardiomyopathy in OVE26 mice via epigenetic regulation of DUSP5-ERK1/2 pathway. Clinical science (London, England : 1979), 131(15), 1841-1857.

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