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Rpl23 16086 1 ap

Manufactured by Proteintech
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RPL23 16086-1-AP is a primary antibody product manufactured by Proteintech. It is designed to detect the RPL23 protein, which is a component of the large ribosomal subunit. This antibody can be used in various immunoassay applications to identify and quantify the RPL23 protein.

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Lab products found in correlation

4 mup alkaline phosphatase substrate, by Thermo Fisher Scientific (2 mentions) Amplex ultrared soluble fluorophore hrp substrate, by Thermo Fisher Scientific (2 mentions) Nanodrop 2000, by Thermo Fisher Scientific (2 mentions) Gstm3, by Abcam (1 mentions) Ab64193, by Abcam (1 mentions) Ab89780, by Abcam (1 mentions) Ab10148, by Abcam (1 mentions) Ab109390, by Abcam (1 mentions) Ab7260, by Abcam (1 mentions)

4 protocols using rpl23 16086 1 ap

1

Quantitative Protein Analysis by Western Blot

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Protein concentrations were determined using a NanoDrop 2000 (Thermo Scientific, NJ, USA). Twenty microgram of tissue lysates in each sample was loaded into polyacrylamide gels for SDS-PAGE, and transferred onto nitrocellulose membranes. The membranes were then blocked with 5% non-fat dry milk in TBS-T (Tris Buffered Saline plus 0.5% Tween) for 60 min and incubated with primary antibodies (ACTB, GTX124213, GeneTex; RPL23, 16086-1-AP, Proteintech; GSTM3, ab74749, Abcam) overnight at 4 °C. Horseradish peroxidase-conjugated secondary antibodies were used to visualize protein bands. Band intensity was analyzed after incubation with ECL reagents, and imaged.
Meng S., Xia W., Pan M., Jia Y., He Z, & Ge W. (2020). Proteomics profiling and pathway analysis of hippocampal aging in rhesus monkeys. BMC Neuroscience, 21, 2.
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2

Immunostaining of Alzheimer's Markers

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Mouse monoclonal or rabbit polyclonal antibodies to tau (ab64193), phospho-Tau (S396) (ab109390), phospho-Tau (T205) (ab4841), ubiquitin (Ubi-1), AβPP (ab15272), Aβ42 (ab10148), SNAP-25 (ab66066), and synaptophysin (ab14692) were purchased from Abeam (Cambridge, MA). Rabbit polyclonal antibody to RPLPO (RPL23 16086-1-AP) was obtained from Proteintech Inc (Chicago, IL). The Amplex UltraRed soluble fluorophore HRP-substrate and 4-MUP alkaline phosphatase substrate were from Invitrogen (Carlsbad, CA). T3D-959 was obtained from T3D Therapeutics, Inc. (Research Triangle Park, NC).
de la Monte S.M., Tong M., Schiano I, & Didsbury J. (2017). Improved Brain Insulin/IGF Signaling and Reduced Neuroinflammation with T3D-959 in an Experimental Model of Sporadic Alzheimer’s Disease. Journal of Alzheimer's disease : JAD, 55(2), 849-864.
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3

Antibody and Substrate Acquisition for Protein Analysis

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Mouse or rabbit polyclonal antibodies to RCAN1 and IDE were purchased from Abcam (Cambridge, MA). Rabbit polyclonal antibody to RPLPO (RPL23 16086-1-AP) was from Proteintech Inc (Chicago, IL). The Amplex UltraRed fluorophore horseradish peroxidase and 4-MUP alkaline phosphatase substrates were obtained from Invitrogen (Carlsbad, CA).
Delikkaya B., Moriel N., Tong M., Gallucci G, & de la Monte S.M. (2019). Altered expression of insulin-degrading enzyme and regulator of calcineurin in the rat intracerebral streptozotocin model and human apolipoprotein E-ε4–associated Alzheimer's disease. Alzheimer's & Dementia : Diagnosis, Assessment & Disease Monitoring, 11, 392-404.
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4

Immunohistochemistry Panel for Neurodegenerative Markers

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Antibodies to tau (ab64193), phospho S396-tau (ab109390), phospho T205-tau (ab4841), ubiquitin (Ubi-1), myelin-associated glycoprotein (MAG-1; ab89780), glial fibrillary acidic protein (GFAP; ab7260), and AβPP-Aβ 1→42 (ab10148) were purchased from Abcam (Cambridge, MA). Rabbit polyclonal antibody to RPLPO (RPL23 16086-1-AP) was purchased from Proteintech Inc (Chicago, IL). Amplex UltraRed soluble fluorophore HRP-substrate, and 4-MUP alkaline phosphatase substrate were purchased from Invitrogen (Carlsbad, CA). T3D-959 was provided by T3D Therapeutics, Inc. (Research Triangle Park, NC).
Tong M., Deochand C., Didsbury J, & de la Monte S.M. (2016). T3D-959: A Multi-Faceted Disease Remedial Drug Candidate for the Treatment of Alzheimer’s Disease. Journal of Alzheimer's disease : JAD, 51(1), 123-138.
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