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Rpl23 16086 1 ap

Manufactured by Proteintech

RPL23 16086-1-AP is a primary antibody product manufactured by Proteintech. It is designed to detect the RPL23 protein, which is a component of the large ribosomal subunit. This antibody can be used in various immunoassay applications to identify and quantify the RPL23 protein.

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4 protocols using rpl23 16086 1 ap

1

Quantitative Protein Analysis by Western Blot

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Protein concentrations were determined using a NanoDrop 2000 (Thermo Scientific, NJ, USA). Twenty microgram of tissue lysates in each sample was loaded into polyacrylamide gels for SDS-PAGE, and transferred onto nitrocellulose membranes. The membranes were then blocked with 5% non-fat dry milk in TBS-T (Tris Buffered Saline plus 0.5% Tween) for 60 min and incubated with primary antibodies (ACTB, GTX124213, GeneTex; RPL23, 16086-1-AP, Proteintech; GSTM3, ab74749, Abcam) overnight at 4 °C. Horseradish peroxidase-conjugated secondary antibodies were used to visualize protein bands. Band intensity was analyzed after incubation with ECL reagents, and imaged.
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2

Immunostaining of Alzheimer's Markers

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Mouse monoclonal or rabbit polyclonal antibodies to tau (ab64193), phospho-Tau (S396) (ab109390), phospho-Tau (T205) (ab4841), ubiquitin (Ubi-1), AβPP (ab15272), Aβ42 (ab10148), SNAP-25 (ab66066), and synaptophysin (ab14692) were purchased from Abeam (Cambridge, MA). Rabbit polyclonal antibody to RPLPO (RPL23 16086-1-AP) was obtained from Proteintech Inc (Chicago, IL). The Amplex UltraRed soluble fluorophore HRP-substrate and 4-MUP alkaline phosphatase substrate were from Invitrogen (Carlsbad, CA). T3D-959 was obtained from T3D Therapeutics, Inc. (Research Triangle Park, NC).
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3

Antibody and Substrate Acquisition for Protein Analysis

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Mouse or rabbit polyclonal antibodies to RCAN1 and IDE were purchased from Abcam (Cambridge, MA). Rabbit polyclonal antibody to RPLPO (RPL23 16086-1-AP) was from Proteintech Inc (Chicago, IL). The Amplex UltraRed fluorophore horseradish peroxidase and 4-MUP alkaline phosphatase substrates were obtained from Invitrogen (Carlsbad, CA).
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4

Immunohistochemistry Panel for Neurodegenerative Markers

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Antibodies to tau (ab64193), phospho S396-tau (ab109390), phospho T205-tau (ab4841), ubiquitin (Ubi-1), myelin-associated glycoprotein (MAG-1; ab89780), glial fibrillary acidic protein (GFAP; ab7260), and AβPP-Aβ 1→42 (ab10148) were purchased from Abcam (Cambridge, MA). Rabbit polyclonal antibody to RPLPO (RPL23 16086-1-AP) was purchased from Proteintech Inc (Chicago, IL). Amplex UltraRed soluble fluorophore HRP-substrate, and 4-MUP alkaline phosphatase substrate were purchased from Invitrogen (Carlsbad, CA). T3D-959 was provided by T3D Therapeutics, Inc. (Research Triangle Park, NC).
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