Kinetex 5 m evo c18 100 mm 4.6 mm

The analysis and detection of phenolic compounds for the five tested plants were carried out according to [22 (link)] with some modifications using Agilent 1260 infinity HPLC Series (Agilent, Santa Clara, CA, USA), equipped with a quaternary pump. The column used was a Kinetex^® 5 µm EVO C18 100 mm × 4.6 mm, (Phenomenex, Torrance, CA, USA) and operated at 30 °C. The separation was conducted using a ternary linear elution gradient with (A) HPLC grade water 0.2% and H₃PO₄ (v/v), (B) methanol and (C) acetonitrile. Then, a volume of 20 µL was injected. AVWD detector set at 284 nm was used for detection of phenols and flavonoids. The analysis was done at the Food Safety and Quality Control Laboratory, Faculty of Agriculture, Cairo University (FSQC 0911-0915/2019).

The extract was analysed, and phenol and flavonoid compounds were detected according to the protocol [44 (link)], where an Agilent 1260 Infinity HPLC (Agilent, USA) was utilized, equipped with a quaternary pump. A Kinetex^® 5 µm EVO C18 100 mm × 4.6 mm (Phenomenex, Santa Clara, CA, USA) column was used, operated at 30 °C. The separation was conducted using a ternary linear elution gradient with (A) HPLC-grade water and 0.2% H₃PO₄ (v/v), (B) methanol, and (C) acetonitrile. Then, 20 µL of the sample was injected, and a variable wavelength detector set at 284 nm was used to recognize phenols and flavonoids. HPLC is selected to convert each component’s area under the curve compared to the area under the standards curve to a concentration (mg/L). In the case of making diluted samples, the concentration is multiplied by the dilution factor. The work was conducted at Cairo University’s Faculty of Agriculture’s Food Safety and Quality Control Laboratory (Reference Number: FSQC0007-21). See Table 1 and Figure 1.
The concentration of phenols and flavonoids are expressed on mg/L. mAu is milli-absorbance unite.

Phenolic compounds were detected in the tested extracts as previously described [47 ], with fine modifications, using an Agilent 1260 infinity HPLC Series (Agilent, Santa Clara, CA, USA) equipped with a quaternary pump. Kinetex^® 5 µm EVO C18 100 mm × 4.6 mm (Phenomenex, Torrance, CA, USA) was used as the column and operated at 30 °C. The separation was carried out using a ternary linear elution gradient with (A) HPLC grade water with 0.2% and H₃PO₄ (v/v), (B) methanol, and (C) acetonitrile. Subsequently, 20 µL of the extract was injected. The AVWD detector (Agilent, Santa Clara, CA, USA) was set at 284 nm to detect phenols and flavonoids.