Human il 21 elisa kits

Manufactured by BioLegend

Sourced in United States

The Human IL-21 ELISA kit is a quantitative immunoassay designed for the measurement of human interleukin-21 (IL-21) in cell culture supernatants, serum, and plasma. The kit utilizes the sandwich ELISA technique to detect and quantify IL-21 levels.

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IL-21 (21 citations) ELISAs (15 citations)

2 protocols using human il 21 elisa kits

IFNγ and IL-21 ELISA Assays

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Human IFNg Uncoated ELISA Kit (Invitrogen, Waltham, MA, USA) and human IL-21 ELISA kits (Biolegend, San Diego, CA, USA) were used for the measurement of IFNg secretion by T cells and IL-21 secretion by BJ feeder cells. For the IFNg secretion assay (as described below), the supernatant was saved after experiments. For IL-21 detection, the supernatant was collected after 3–5 day of BJ feeder cell culturing. The ELISA procedures were performed according to the manufacturer’s instructions. Experiments were carried out either in duplicate or triplicate.

Wang Z., Zhang T., Anderson A., Lee V., Szymura S., Dong Z., Kuang B., Oh E., Liu J., Neelapu S.S., Kwak L, & Cha S.C. (2023). Immortalized B Cells Transfected with mRNA of Antigen Fused to MITD (IBMAM): An Effective Tool for Antigen-Specific T-Cell Expansion and TCR Validation. Biomedicines, 11(3), 796.

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Measuring Serum IL-21 via ELISA

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Serum IL-21 concentrations were measured using human IL-21 ELISA kits (Biolegend, San Diego, CA, USA), according to the manufacturer’s instructions.

Wang X., Li T., Si R., Chen J., Qu Z, & Jiang Y. (2020). Increased frequency of PD-1hiCXCR5- T cells and B cells in patients with newly diagnosed IgA nephropathy. Scientific Reports, 10, 492.

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