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Vectastain peroxidase abc kit 6100

Manufactured by Vector Laboratories
Sourced in Germany

The Vectastain Peroxidase ABC-kit 6100 is a reagent kit designed for use in immunohistochemical staining procedures. The kit contains the necessary components for the detection of target antigens in tissue sections or cell preparations using the avidin-biotin complex (ABC) method.

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2 protocols using vectastain peroxidase abc kit 6100

1

Immunohistochemical Analysis of Pancreatic Islets

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Sections were deparaffinized and heated in citrate buffer for improved antigen retrieval and further incubated with anti‐islet‐1/2 (40.2D6) 1:50; anti‐gag (AMV3C2, 1:200; both antibodies from Developmental Studies Hybridoma Bank, University of Iowa), anti‐somatostatin (Millipore; 1:100); anti‐BrdU (Sigma; 1:1000); anti‐active caspase 3 (R&D; 1:500) and visualized using biotinylated secondary antibodies (donkey‐anti‐mouse; –anti‐rabbit; ‐anti‐rat; 1:100; Dianova, Hamburg, Germany) and diaminobenzidine (Vectastain Peroxidase ABC‐kit 6100, Vector Laboratories, CA, USA).
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2

Immunohistochemical Analysis of Pancreatic Islets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sections were de-paraffinized and heated in citrate buffer for improved antigen retrieval and further incubated with anti-islet-1/2 (40.2D6) 1:50; anti-gag (AMV3C2, 1:200; both from Developmental Studies Hybridoma Bank, University of Iowa), anti-BrdU (Sigma; 1:1000); anti-active-caspase-3 (R&D; 1:500) and visualized using biotinylated secondary antibodies (donkey-anti-mouse; -anti-rabbit; -anti-rat; 1:100; Dianova, Hamburg, Germany) and diaminobenzidine (Vectastain Peroxidase ABC-kit 6100, Vector Laboratories, CA, United States).
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