Dexamethasone (dex)

CAA was dissolved in dimethyl sulfoxide (DMSO, Sigma, St. Louis, MO, U.S.A.) to a concentration of 200 μM and stored at −20°C as a stock solution. The stock solution was diluted with Dulbecco’s modified Eagle’s medium (DMEM, HyClone, U.S.A) to a final concentration of 50 μM before use. Dexamethasone (Cell Signaling Technology, #9668) was diluted with DMEM to a final concentration of 1 μM. DMSO was added in the vehicle control group. TGF-β (240-B; R&D Systems, MN, U.S.A.) was diluted to a concentration of 6.4 ng/ml, and IL-6 (206-IL/CF, R&D Systems, MN, U.S.A.) was diluted to a concentration of 8 ng/ml before use. Insulin (Cell Signaling Technology, #9668), human IL-6 high sensitivity enzyme-linked immunosorbent assay (ELISA) Kit (Abcam, ab46042, U.S.A), human IL-17 High Sensitivity ELISA Kit (Abcam, ab46042, U.S.A), propidium iodide (Cell Signaling Technology, #9668), 4′,6-diamidino-2-phenylindole (DAPI; Cell Signaling Technology, #9668), primary antibodies including anti-IL-17RC, anti-IL-6, anti-p-IR (Cell Signaling Technology, #9542), anti-GLUT1 (Cell Signaling Technology, #9542), and monoclonal mouse anti-GAPDH (Santa Cruz, CA, U.S.A.), and horseradish peroxidase (HRP)–conjugated polyclonal goat anti-mouse and anti-rabbit (both from Santa Cruz, CA, U.S.A.) were used in the present study. Human IL-17RC adenovirus was purchased from Vector Biolabs (Malvern, PA, U.S.A.).

Acetic acid, acrylamide, bis-acrylamide, agarose, ANX (anisomycin), Brij-35, bromophenol blue, calcium chloride, cisplatin, Coomassie Brilliant Blue, crystal violet, DAPI (4′,6-diamidino-2-phenylindole dihydrochloride), DMSO (dimethyl sulfoxide), EDTA, 5-fluorouracil (5-FU), GA, gelatin, glycerol, glycine, MAG, methanol, paraformaldehyde, potassium hexacyano-ferrate (II) trihydrate, potassium hexacyano-ferrate (III), potassium phosphate monobasic (KH₂PO₄), potassium phosphate dibasic (K₂HPO₄), sodium dodecyl sulfate, sodium chloride, Tris, triton X-100, and zinc sulfate were purchased from Sigma-Aldrich. Bradford reagent was purchased from Fermentas. All media and chemicals used for in vitro cell cultivation (DMEM, FBS, Ham’s F-12, McCoy’s 5a, Dulbecco’s phosphate-buffered saline (D-PBS), trypsin-EDTA, gentamicin, hydrocortisone), CellTrace™ CFSE Cell Proliferation Kit, human recombinant epidermal growth factor (EGF), Gel-Red, and Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix Gibco™ were purchased from ThermoFisher Scientific (Waltham, MA, USA). Matrigel^® (BD Matrigel Cellware Basement Membrane) was purchased from Becton-Dickinson, Erembodegem, Belgium. Camptothecin and dexamethasone were purchased from Cell Signaling Technology.