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Anti hla dm pe

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Anti-HLA-DM-PE is a fluorescently labeled antibody that binds to HLA-DM, a protein involved in the antigen presentation pathway. This product can be used for flow cytometric analysis and other immunoassays.

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Lab products found in correlation

Anti cd80 bv421, by BioLegend (2 mentions) Pnl nlucp nfat re hygro, by Promega (2 mentions)

2 protocols using anti hla dm pe

1

Generating NFAT Reporter T-cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Jurkat 76 T-cell line, deficient for both TCRα and TCRβ chains, was kindly provided by Dr. Shao-An Xue (Department of Immunology, University College London). The NFAT reporter cell line (J76-NFATRE-luc) was constructed using lentiviral transfer of pNL[NlucP/NFAT-RE/Hygro] (Promega) into the Jurkat 76 cell line. Single cell clones with the best fold induction were selected based on the induction of luciferase after PMA/Ionomycin stimulation. K562 cell line was obtained from the ATCC and cultured under standard conditions. Artificial antigen presenting cells (aAPC) were constructed using lentiviral transduction of CD80, HLA-DM molecules and different HLA alleles (gBlock ordered from IDT) into K562 cells. The surface expression of CD80 and HLA-DM on K562 was confirmed using anti-CD80-BV421 and anti-HLA-DM-PE antibodies from BioLegend.
Huang H., Wang C., Rubelt F., Scriba T.J, & Davis M.M. (2020). Analyzing the M. tuberculosis immune response by T cell receptor clustering with GLIPH2 and genome-wide antigen screening. Nature biotechnology, 38(10), 1194-1202.
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2

Generating NFAT Reporter T-cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Jurkat 76 T-cell line, deficient for both TCRα and TCRβ chains, was kindly provided by Dr. Shao-An Xue (Department of Immunology, University College London). The NFAT reporter cell line (J76-NFATRE-luc) was constructed using lentiviral transfer of pNL[NlucP/NFAT-RE/Hygro] (Promega) into the Jurkat 76 cell line. Single cell clones with the best fold induction were selected based on the induction of luciferase after PMA/Ionomycin stimulation. K562 cell line was obtained from the ATCC and cultured under standard conditions. Artificial antigen presenting cells (aAPC) were constructed using lentiviral transduction of CD80, HLA-DM molecules and different HLA alleles (gBlock ordered from IDT) into K562 cells. The surface expression of CD80 and HLA-DM on K562 was confirmed using anti-CD80-BV421 and anti-HLA-DM-PE antibodies from BioLegend.
Huang H., Wang C., Rubelt F., Scriba T.J, & Davis M.M. (2020). Analyzing the M. tuberculosis immune response by T cell receptor clustering with GLIPH2 and genome-wide antigen screening. Nature biotechnology, 38(10), 1194-1202.
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