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Cd45 buv95

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The CD45 BUV95 is a fluorochrome-conjugated antibody that binds to the CD45 antigen, which is a marker expressed on the surface of various hematopoietic cells, including lymphocytes, monocytes, and granulocytes. The BUV95 fluorochrome provides a specific emission spectrum that can be detected using flow cytometry.

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Lab products found in correlation

Cd3 apc cy7, by BioLegend (2 mentions) Cd19 bv605, by BioLegend (2 mentions) Nk1.1 pe cy7, by BioLegend (2 mentions) Fortessa x20, by BD (2 mentions) Ly6c pacific blue, by BioLegend (2 mentions) Ly6b fitc, by Abcam (2 mentions) Mhc class 2 a700, by BioLegend (2 mentions) Cd32b apc, by Thermo Fisher Scientific (2 mentions) Cd16.2 apc, by BioLegend (2 mentions) Cd16 fitc, by BioLegend (2 mentions) Cd64 apc, by BioLegend (2 mentions) Ly6g pe cy7, by BioLegend (2 mentions) Cd11b percp cy5, by BioLegend (2 mentions) Macsquant analyzer, by Miltenyi Biotec (2 mentions) Ethylene diamine tetraacetic acid (edta), by Corning (2 mentions) Cd45 buv395, by BD (2 mentions)

2 protocols using cd45 buv95

1

Isolation and Staining of Myeloid Cells

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For cell depletion experiments, whole blood was harvested from mice and mixed with 5 mM EDTA (Corning). Red blood cells were lysed with ACK lysis buffer at room temperature before being washed and chilled in PBS + 5% FCS. Monocytes were stained with CD45 BUV395 (BD Biosciences clone 30-F11), CD11b PerCP-Cy5.5 (BioLegend clone M1/70), Ly6B FITC (Abcam clone 7/4), Ly6G PE-Cy7 (BioLegend clone 6D5), Ly6C Pacific Blue (BioLegend clone HK1.4) and MHC class II A700 (BioLegend clone M5/114.15.2). NK cells were stained with CD45 BUV95, CD3 APC-Cy-7 (BioLegend clone 145–2C11), CD19 BV605 (BioLegend clone 6D5), and NK1.1 Pe-Cy7 (BioLegend clone PK136). Viability was determined through exclusion of a fixable viability dye (e506;eBiosciences). Samples were fixed and processed on a BD-Fortessa X20. For FcγR expression experiments BV2, LADMAC, and Vero cells were stained with one of the following: CD64 APC (BioLegend clone X54–5/7.1), CD32b APC (Invitrogen clone AT130–2), CD16 FITC (BioLegend clone 221–3A4), CD16.2 APC (BioLegend clone 9E9). Cells were analyzed on a MACSQuant analyzer (Miltenyi). All FACS data were analyzed by FlowJo v. 10.7.
Earnest J.T., Holmes A.C., Basore K., Mack M., Fremont D.H, & Diamond M.S. (2021). The mechanistic basis of protection by non-neutralizing anti-alphavirus antibodies. Cell reports, 35(1), 108962.
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2

Isolation and Staining of Myeloid Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For cell depletion experiments, whole blood was harvested from mice and mixed with 5 mM EDTA (Corning). Red blood cells were lysed with ACK lysis buffer at room temperature before being washed and chilled in PBS + 5% FCS. Monocytes were stained with CD45 BUV395 (BD Biosciences clone 30-F11), CD11b PerCP-Cy5.5 (BioLegend clone M1/70), Ly6B FITC (Abcam clone 7/4), Ly6G PE-Cy7 (BioLegend clone 6D5), Ly6C Pacific Blue (BioLegend clone HK1.4) and MHC class II A700 (BioLegend clone M5/114.15.2). NK cells were stained with CD45 BUV95, CD3 APC-Cy-7 (BioLegend clone 145–2C11), CD19 BV605 (BioLegend clone 6D5), and NK1.1 Pe-Cy7 (BioLegend clone PK136). Viability was determined through exclusion of a fixable viability dye (e506;eBiosciences). Samples were fixed and processed on a BD-Fortessa X20. For FcγR expression experiments BV2, LADMAC, and Vero cells were stained with one of the following: CD64 APC (BioLegend clone X54–5/7.1), CD32b APC (Invitrogen clone AT130–2), CD16 FITC (BioLegend clone 221–3A4), CD16.2 APC (BioLegend clone 9E9). Cells were analyzed on a MACSQuant analyzer (Miltenyi). All FACS data were analyzed by FlowJo v. 10.7.
Earnest J.T., Holmes A.C., Basore K., Mack M., Fremont D.H, & Diamond M.S. (2021). The mechanistic basis of protection by non-neutralizing anti-alphavirus antibodies. Cell reports, 35(1), 108962.
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