Log In Sign up
The largest database of trusted experimental protocols

Ucp1 antibody ab10983

Manufactured by Abcam
Visit Supplier
Sourced in United Kingdom

The UCP1 antibody (ab10983) is a tool for the detection and quantification of the UCP1 protein, which is a key regulator of thermogenesis in brown adipose tissue. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunofluorescence.

Automatically generated - may contain errors

Lab products found in correlation

Dapi solution, by Dojindo Laboratories (1 mentions) Lipi red, by Dojindo Laboratories (1 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Bz x710 all in one fluorescence microscope, by Keyence (1 mentions) Ro4929097, by Selleck Chemicals (1 mentions) Cleaved caspase 3 9661, by Cell Signaling Technology (1 mentions) Ly450139, by Selleck Chemicals (1 mentions) Pf 03084014, by Selleck Chemicals (1 mentions) Ly3039478, by Selleck Chemicals (1 mentions) Anti rabbit igg 7074, by Cell Signaling Technology (1 mentions) Pparγ 81b8, by Cell Signaling Technology (1 mentions) Anti mouse igg 7076, by Cell Signaling Technology (1 mentions) β actin sc 47778, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

UCP1 (ab10983, UCP1 antibody Ab10983

2 protocols using ucp1 antibody ab10983

1

Mitochondrial Staining and UCP1 Expression

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunocytochemistry was performed as previously described21 (link). In brief, differentiated and undifferentiated AdMSCs were incubated with either 250 nM MitoTracker Red CMXRos (ThermoFisher Scientific) or 1 µM Lipi-Red (Dojindo, Kumamoto, Japan) for 30 min at 37 °C in 5% CO2, according to the manufacturer’s instructions. Then the cells were fixed with 4% paraformaldehyde for 10 min. After washing with PBS, the cells were incubated with PBS containing 0.1% Triton X-100 for 5 min. They were blocked with PBS containing 3% skim milk for 1 h at room temperature. The cells were incubated with UCP1 antibody (ab10983, Abcam, Cambridge, UK) at 1/1000 dilution overnight at 4 °C. After washing with PBS, the cells were further incubated with Alexa Fluor 488 donkey anti-rabbit IgG (ThermoFisher Scientific) for 1 h at room temperature. Cell nuclei were stained with DAPI solution (Dojindo, Kumamoto, Japan). All images were obtained using a BZ-X710-All-in-One Fluorescence Microscope (Keyence, Osaka, Japan). All scale bars represent 100 μm.
Takeda Y., Yoshikawa T, & Dai P. (2021). Transcriptome analysis reveals brown adipogenic reprogramming in chemical compound-induced brown adipocytes converted from human dermal fibroblasts. Scientific Reports, 11, 5061.
+ Open protocol
+ Expand
2

Screening Modulators of Stemness Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
MK-0752 was purchased from APExBIO (Houston, TX, USA). RO4929097, PF-03084014, LY3039478, and LY450139 were obtained from Selleckchem (Houston, TX, USA). BMS-708163 and BMS-906024 were purchased from Tocris Bioscience (Bristol, UK) and MilliporeSigma (Burlington, MA, USA), respectively. CellTiter 96® AQueous one solution reagent containing a tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] was purchased from Promega (Madison, WI, USA). Propidium iodide (P4864) was purchased from Sigma-Aldrich (St. Louis, MO, USA). UCP1 antibody (ab10983) and goat polyclonal secondary antibody to rabbit immunoglobulin G (IgG) - H&L (Alexa Fluor® 488, ab150077) were obtained from Abcam (Cambridge, UK). CCAAT/enhancer-binding protein alpha (C/EBPα, SC-61), and β-Actin (SC-47778) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Cleaved caspase-3 (9661S), peroxisome proliferator-activated receptor gamma (PPARγ, 81B8), and horseradish peroxidase (HRP)-conjugated secondary antibodies, including anti-rabbit IgG (7074S) and anti-mouse IgG (7076S), were purchased from Cell Signaling Technology (Danvers, MA, USA). All other reagents and solvents were purchased from Sigma-Aldrich.
Huang D., Qiu J., Kuang S, & Deng M. (2020). In Vitro Evaluation of Clinical Candidates of γ-Secretase Inhibitors: Effects on Notch Inhibition and Promoting Beige Adipogenesis and Mitochondrial Biogenesis. Pharmaceutical research, 37(10), 185.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!