For all other serum analyses, submandibular blood collection was performed immediately prior to sacrifice and serum was separated. Insulin ELISA (Millipore #EZRMI-13K), triglycerides (Thermo Fisher Scientific #TR22421), cholesterol (Thermo Fisher Scientific #TR13421), and free fatty acids (Wako Diagnostics #999-34691, #995-34791, #991-34891, #993-35191) quantification were performed using commercially available reagents according to manufacturer’s directions. Albumin levels were quantified using an AMS LIASYS Chemistry Analyzer.
Ezrmi 13k
Manufactured by Thermo Fisher Scientific
The EZRMI-13K is a laboratory instrument designed for the detection and quantification of microorganisms in environmental and food samples. The core function of this product is to provide accurate and reliable results through rapid microbial identification and enumeration.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using ezrmi 13k
1
Serum Biomarker Analysis Protocol
2
Metabolic Biomarker Profiling Protocol
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Fasting blood glucose was measured via glucometer using tail vein blood. For all other serum analyses, submandibular blood collection was performed immediately prior to sacrifice, and serum was separated. Insulin ELISA (MilliporeSigma, catalog EZRMI-13K), TG (Thermo Fisher Scientific, catalog TR22421), cholesterol (Thermo Fisher Scientific, catalog TR13421), and free FA (FFA) (Wako Diagnostics, catalog 999-34691, 995-34791, 991-34891, and 993-35191) quantification was performed using commercially available reagents according to manufacturer’s directions. Albumin levels were quantified using an AMS LIASYS Chemistry Analyzer.
3
Comprehensive Serum and Hepatic Lipid Analysis
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For all other serum analyses, submandibular blood collection was performed immediately prior to sacrifice and serum was separated. Insulin ELISA (Millipore #EZRMI-13K), triglycerides (Thermo Fisher Scientific #TR22421), cholesterol (Thermo Fisher Scientific #TR13421), and free fatty acids (Wako Diagnostics #999-34691, #995-34791, #991-34891, #993-35191) quantification were performed using commercially available reagents according to manufacturer’s directions. Albumin levels were quantified using an AMS LIASYS Chemistry Analyzer.
Hepatic lipids were extracted from ~100 mg hepatic tissue homogenized in 2:1 chloroform:methanol. In total, 0.25–0.5% of each extract was evaporated overnight prior to biochemical quantification of triglycerides, LDL-C, cholesterol, and free fatty acids using reagents described above, precisely according to manufacturer’s directions.
Hepatic lipids were extracted from ~100 mg hepatic tissue homogenized in 2:1 chloroform:methanol. In total, 0.25–0.5% of each extract was evaporated overnight prior to biochemical quantification of triglycerides, LDL-C, cholesterol, and free fatty acids using reagents described above, precisely according to manufacturer’s directions.
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