Mh7a synovial cells

SW1353 and OUMS-27 human CS cells, C28/I2 chondrocytes (obtained from Dr. M. Goldring, Hospital for Special Surgery, New York, NY, USA), and 4T1.2 mouse mammary tumor cells (obtained from Dr. R. Anderson at Peter MacCallum Cancer Institute, Melbourne, Australia) were cultured in DMEM [26 (link),27 (link),28 (link),29 (link)] RAW264.7 pre-osteoclast cells (ATCC, Manassas, VA, USA) were grown in αMEM [30 (link)]. Human MSCs (Lonza, Basel, Switzerland) were grown in MSC Basal Medium (Lonza). MH7A synovial cells (Riken Cell Bank, Tsukuba, Japan) were cultured in RPMI1640 [31 (link)]. The culture media were supplemented with 10% FBS (fetal bovine serum) and antibiotics (penicillin and streptomycin), and cells were maintained at 37 °C and 5% CO₂.

MH7A synovial cells isolated from intra-articular soft tissues of the knee joints of RA patients were obtained from Riken Cell Bank (Saitama, Japan). MH7A is a cell line established by transfecting with the SV40 T antigen (Miyazawa et al. 1988 (link)). MH7A cells were cultured in RPMI 1640 (Sigma, St. Louis, MO) containing 10% heat-inactivated fetal bovine serum (FBS) (Whittaker, Walkersville, MD), 100 units/ml of penicillin and 100 μg/ml of streptomycin (Invitrogen, San Diego, CA) at 37°C in an atmosphere of 5% CO₂ in air.