LPO was assayed with Lipid Hydroperoxide ELISA assay kit (Cayman Chemical Company, Ann Arbor, MI, USA) by direct measurement of redox reaction with iron ions according to the kit manual. Briefly, in 500 µL test samples, the solution “LPO Assay Extract R” (part of OxiSelect AOPP ELISA assay kit) was added. To this mixture, 1 mL of cold chloroform solution was added, blended, and centrifuged. A total of 450 µL of mixture chloroform-methanol was added to 500 µL of extract chloroform-sample, followed by a 50 µL mixture of chromogen, which turned purple. The absorbance was measured at 500 nm with a Spectro UVD-3500 spectrophotometer (Labomed Inc, Los Angeles, CA, USA). From the calibration curve of LPO, we calculated the concentration of LPO in each sample according to the formula specified by the manufacturer and expressed as µM.
Spectro uvd 3500 spectrophotometer
Manufactured by Labomed
Sourced in United States
The Spectro UVD-3500 is a spectrophotometer that measures the absorbance or transmittance of light in the ultraviolet and visible wavelength ranges. It is designed to analyze the composition and concentration of various samples by detecting the amount of light absorbed or transmitted at specific wavelengths.
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Lab products found in correlation
2 protocols using spectro uvd 3500 spectrophotometer
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Lipid Hydroperoxide ELISA Assay
2
Quantifying Lipid Hydroperoxide Levels
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Lipid hydroperoxide was assayed with the Lipid Hydroperoxide Assay kit (Cayman Chemical Company) by direct measurement of redox reaction with iron ions according to the kit manual. Briefly, the solution LPO Assay Extract R was added to test samples. To this mixture, 1 ml cold chloroform solution was added and blended. A total of 450 µl of the chloroform-methanol mixture was added to 500 µl of extract chloroform sample, followed by a 50 µl mixture of chromogen, which turned purple. Absorbance was measured at 500 nm with a Spectro UVD-3500 spectrophotometer (Labomed Inc., Los Angeles, CA). From the calibration curve of LPO, we calculated the concentration of LPO in each sample according to the formula specified by the manufacturer and expressed as µM.
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