Bacto tryptone

The bacterial strains and plasmids used in this study are listed in Table 1. Wild-type S. Enteritidis P125109 strain and its isogenic mutants were grown in Luria–Bertani (LB) medium (10 g/l Bacto tryptone, 5 g/l Bacto yeast extract, 5 g/l NaCl, HiMedia, India), at 37 °C. For invasion experiments, bacterial strains were grown in SPI1 inducing environment (LB containing 0.3 M NaCl) at 120 rpm. Antibiotics in growth media were used at the following concentrations: ampicillin, 100 µg/ml; kanamycin, 50 µg/ml; and streptomycin, 50 µg/ml.

The bacterial strains and plasmids used in this study have been listed in Tables 1 and 2 respectively. Wild type S. Typhimurium SB30035 (link) and its isogenic mutants were grown in Luria Bertani (LB) medium (10 g/L Bacto tryptone, 5 g/L Bacto yeast extract, 5 g/L NaCl, HiMedia, India) or minimal E glucose medium (minimal EG) containing MgSO₄.7H₂O (200 mg), citric acid.H₂O (2 g), K₂HPO₄ (10 g), NaH₂PO₄ (1.75 g), (NH₄)₂HPO₄ (1.75 g) in 1 L of Milli-Q water, adjusted to pH 7.536 (link). The minimal medium was supplemented with 0.4% dextrose and 0.1% casein hydrolysate (both 0.22 μm filtered). Antibiotics in growth media were used at the following concentrations: ampicillin 100 μg/ml, kanamycin 50 μg/ml, streptomycin 50 μg/ml and chloramphenicol 20 μg/ml. Bacterial strains were grown at 37 °C in a shaking incubator at 150 rpm.