Aav2 1 hsyn flex chr2 eyfp

The following viruses and were used: AAV2/1-hSyn-flex-ChR2-eYFP (Addgene), AAV-pgk-retro-Cre (Addgene), AAV-flex-GFP (Addgene), AAV-flex-tdTomato (Addgene), AAV-flex-GCamp8s (Addgene), and AAV-flex-oG (Duke Viral Vector Core). EnvA-ΔG-RV-GFP and AAV-flex-TVA-mCherry were produced in house as previously described (Rodriguez et al., 2017 (link); Sakurai et al., 2016 (link); Tschida et al., 2019 (link); Michael et al., 2020 (link)). The final injection coordinates were as follows: POA, AP = 0.14 mm, ML = 0.3 mm, DV = 5.5 mm; Amg_C/M, AP = −1.5 mm, ML = 2.3 mm, DV = 4.6 mm; PAG, AP = −4.7 mm, ML = 0.7 mm, DV = 1.75 mm. Viruses were pressure-injected with a Nanoject II (Drummond) at a rate of 4.6 nL every 15 s.

The following viruses and injection volumes were used: AAV2/1-hSyn-FLEX-ChR2-eYFP (Addgene), AAV-pgk-retro-Cre (Addgene), AAV-hsyn-retro-FLEX-ChR2 (Addgene), AAV-FLEX-GFP (Addgene), AAV-FLEX-tdTomato (Addgene), AAV-FLEX-oG (Duke Viral Vector Core). EnvA-ΔG-RV-GFP, CANE-RV-mCherry, and AAV-FLEX-TVA-mCherry were produced in house as previously described (Rodriguez et al., 2017 (link); Sakurai et al., 2016 (link); Tschida et al., 2019 (link)). The final injection coordinates were as follows: POA, AP = 0.14 mm, ML = 0.3 mm, DV = 5.5 mm; Amg_C/M, AP = −1.5 mm, ML = 2.3 mm, DV = 4.6 mm; PAG, AP = −4.7 mm, ML = 0.7 mm, DV = 1.75 mm. Viruses were pressure-injected with a Nanoject II (Drummond) at a rate of 4.6 nL every 15 s.