Two mice in each group with body weights similar to the median were selected as representative mice. A minimum of five tissue sites was randomly selected for imaging, and a representative image is presented in the figures. Samples of TA muscle were fixed with 2% glutaraldehyde in paraformaldehyde (Merck & Co. Inc., Kenilworth, NJ, USA) overnight at 4°C and post-fixed for 1 hour in 1% OsO4 resin (Polysciences Inc., Warrington, PA, USA). Samples were dehydrated in ethanol (Merck & Co.), embedded in rubber molds with epoxy resin (Polysciences Inc.), and polymerized in an oven at 60°C for 20 hours. Semithin sections (1 μm thick) were cut using a Leica Ultracut UCT (Leica Microsystems Inc., Buffalo Grove, IL USA) and stained with toluidine blue (Merck). Ultrathin sections (70 nm thick) were cut and mounted on coated copper grids (Nisshin EM, Tokyo, Japan) and double stained with 6% uranyl acetate (Ted Pella Inc., Redding, CA, USA) and lead citrate (Wako, Osaka, Japan). The ultrastructure of the tissue sections was observed using a JEM-1011 transmission electron microscope (TEM, JEOL, Tokyo, Japan) with an accelerating voltage of 80 Kv. Images were viewed using Camera-Megaview III software (EMSIS, Munster, Germany).
Lead citrate
Manufactured by Fujifilm
Sourced in United States, Japan
Lead citrate is a chemical compound used in the preparation of samples for electron microscopy. It functions as a stain, enhancing the contrast of biological specimens to improve visualization during microscopic analysis.
Automatically generated - may contain errors
Lab products found in correlation
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Toluidine blue, by Merck Group (1 mentions)
Ultracut uct, by Leica (1 mentions)
Uranyl acetate, by Ted Pella (1 mentions)
Jem 1011 transmission electron microscope, by JEOL (1 mentions)
Epoxy resin, by Polysciences (1 mentions)
Ultracut uct microtome, by Leica (1 mentions)
H 7650 instrument, by Hitachi (1 mentions)
Uranyl acetate, by Fujifilm (1 mentions)
Lr white resin, by Agar Scientific (1 mentions)
2 protocols using lead citrate
1
Ultrastructural Analysis of Mouse Skeletal Muscle
2
Transmission Electron Microscopy of EV Pellets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The EV pellets collected at the bottom of tubes were directly fixed with 100 μL of modified Karnovsky’s fixative solution80 (2.0% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.2) for 1 h at 4 °C. After fixation, the pellets were carefully recovered with a spatula and embedded in LR White resin (Agar Scientific, Stansted, Essex, LDN, UK). Ultra-thin sections were prepared with an Ultracut UCT microtome (S9329, Leica, S9329, Wetzlar, Germany) and the specimens were stained with 6% uranyl acetate (Wako, Tokyo, Japan) and 3% lead citrate (Wako) on formvar-coated (Nissin EM, Tokyo, Japan) nickel grids (S-300 square mash, Gilder, Grantham, UK). Transmission electron microscopy (TEM) images were obtained with a H-7650 instrument (Hitachi, Co., Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!