The flag-tagged coding sequence of DARPP-32, SRp20 and CD44E were cloned in pcDNA3.1 mammalian expression plasmid (Invitrogen). AGS cells stably expressing DARPP-32 or pcDNA3.1 empty vector were generated as described previously (Belkhiri et al 2005 (link), El-Rifai et al 2002 (link)). Lentivirus particles expressing DARPP-32 shRNA or control shRNA were produced by GeneCopoeia (Rockville, MD) and then utilized to transduce MKN-45 cells. SRp20 siRNA (sc-38338) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA).
Srp20 sirna sc 38338
Manufactured by Santa Cruz Biotechnology
SRp20 siRNA (sc-38338) is a small interfering RNA (siRNA) product designed to target the expression of the SRp20 (serine/arginine-rich splicing factor 20) gene. The SRp20 protein is involved in pre-mRNA splicing and regulates alternative splicing. This siRNA product is intended for research use only and its core function is to knockdown or silence the expression of the SRp20 gene in various cell-based experimental systems.
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Lab products found in correlation
2 protocols using srp20 sirna sc 38338
1
Molecular Cloning and Knockdown Approaches
2
Molecular Cloning and Knockdown Approaches
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The flag-tagged coding sequence of DARPP-32, SRp20 and CD44E were cloned in pcDNA3.1 mammalian expression plasmid (Invitrogen). AGS cells stably expressing DARPP-32 or pcDNA3.1 empty vector were generated as described previously (Belkhiri et al 2005 (link), El-Rifai et al 2002 (link)). Lentivirus particles expressing DARPP-32 shRNA or control shRNA were produced by GeneCopoeia (Rockville, MD) and then utilized to transduce MKN-45 cells. SRp20 siRNA (sc-38338) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA).
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