Whole-cell lysates were prepared from cultured HCAECs with radioimmunoprecipitation assay buffer (Santa Cruz Biotechnology, Inc.), and the lysates were then separated via 10% SDS-PAGE, transferred to polyvinylidene fluoride membranes, and probed with antibodies: Rabbit anti-Jagged1 (1:1,000; cat. no. ab109536, Abcam), rabbit anti-IGFBP1 (1:1,000; cat. no. ab181141; Abcam), rabbit anti-Akt (1:1,000; cat. no. 4691, Cell Signaling Technology, Inc.), rabbit anti phosphorylated (p)-Akt (1:1,000; cat. no. 4060, Cell Signaling Technology, Inc.) and rabbit anti-βactin (1:1,000; cat. no. ab8227; Abcam) by standard procedures.
Rabbit anti jagged 1
Manufactured by Santa Cruz Biotechnology
Sourced in United Kingdom
Rabbit anti-Jagged 1 is a primary antibody that specifically binds to the Jagged 1 protein. Jagged 1 is a cell surface protein involved in the Notch signaling pathway, which plays a crucial role in cell-cell communication and developmental processes.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 880 inverted confocal microscope, by Zeiss (2 mentions)
Rabbit anti sox2, by Abcam (2 mentions)
Mouse igg1 monoclonal anti sox2, by BD (2 mentions)
Mouse igg1 anti islet1, by Developmental Studies Hybridoma Bank (2 mentions)
Mouse igg1 anti myo7a, by Developmental Studies Hybridoma Bank (2 mentions)
Mouse igg1 anti hca, by Developmental Studies Hybridoma Bank (2 mentions)
Rabbit anti akt, by Abcam (1 mentions)
Radioimmunoprecipitation assay buffer, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti β actin, by Cell Signaling Technology (1 mentions)
Rabbit anti igfbp1, by Abcam (1 mentions)
Alexa dye, by Thermo Fisher Scientific (1 mentions)
3 protocols using rabbit anti jagged 1
1
Western Blot Analysis of HCAEC Signaling
2
Whole-mount Immunostaining of Chicken Embryos
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Entire chicken embryos (E3–E4) or their heads (>E5) were collected, fixed for 1.5–2 hr in 4% paraformaldehyde (PFA) in 0.1 M phosphate buffered saline (PBS), and processed for whole-mount immunostaining using conventional methods. Further details of the protocol and reagents can be found in the Appendix 1 file. The following antibodies were used: rabbit anti-Jagged 1 (Santa-Cruz Biotechnology, Dallas, TX; sc-8303; 1:200), rabbit anti-Sox2 (Abcam, UK; 97959, 1:500), mouse IgG1 monoclonal anti-Sox2 (BD Biosciences, San Jose, CA; 561469, 1:500), mouse IgG1 anti-Islet1 (Developmental Studies Hybridoma Bank, Iowa City, IA; Clone 39.3F7, 1:250), mouse IgG1 anti-HA-tag (Babco Inc, Richmond, CA; MMS-101R, 1:500), mouse IgG1 anti-Myo7a (Developmental Studies Hybridoma Bank, 1:500), and mouse IgG1 anti-HCA (a kind gift of Guy Richardson, 1:1000). Secondary goat antibodies conjugated to Alexa dyes (1:1000) were obtained from Thermo Fischer Scientific (UK). Confocal stacks were acquired using a Zeiss LSM880 inverted confocal microscope and further processed with ImageJ.
3
Immunostaining Embryonic Chicken Tissues
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Entire chicken embryos (E3-E4) or their heads (>E5) were collected, fixed for 1.5-2h in 4% immunostaining using conventional methods. Further details of the protocol and reagents can be found in the Supplementary Material and Methods file. The following antibodies were used: rabbit anti-Jagged 1 (Santa-Cruz Biotechnology, Dallas, TX; sc-8303; 1:200), rabbit anti-Sox2 (Abcam, UK; 97959, 1:500), mouse IgG1 monoclonal anti-Sox2 (BD Biosciences, San Jose, CA; 561469, 1:500), mouse IgG1 anti-Islet1 (Developmental Studies Hybridoma Bank, Iowa City, IA; Clone 39.3F7, 1:250), mouse IgG1 anti-HA-tag (BabcoInc., Richmond, CA; MMS-101R, 1:500), mouse IgG1 anti-Myo7a (Developmental Studies Hybridoma Bank, 1:500), mouse IgG1 anti-HCA (a kind gift of Guy Richardson, 1:1000). Secondary goat antibodies conjugated to Alexa dyes (1:1000) were obtained from Thermo Fischer Scientific (UK). Confocal stacks were acquired using a Zeiss LSM880 inverted confocal microscope and further processed with ImageJ.
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