Quantification of immunoblots was performed by densitometric analysis using the Image J software or the LI-COR Biosciences infrared imager software and normalized to loading control (actin or tubulin, as indicated). The p-values were determined by two-tailed Student’s t-test.
Aggregates were counted in at least 200 cells per slide (with the observer blinded to their identity), and percentage was calculated relative to control conditions. p-values were determined by unpaired two-tailed Student’s t-test.
All experiments were done at least three times in triplicate and a representative blot or graph from a triplicate experiment is shown unless indicated.
Aggregates were counted in at least 200 cells per slide (with the observer blinded to their identity), and percentage was calculated relative to control conditions. p-values were determined by unpaired two-tailed Student’s t-test.
All experiments were done at least three times in triplicate and a representative blot or graph from a triplicate experiment is shown unless indicated.