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Horseradish peroxidase hrp conjugated anti mouse or anti rat antibodies

Manufactured by Southern Biotech
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Horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rat antibodies are laboratory reagents used in various immunoassay techniques. The HRP enzyme is covalently linked to the antibody, enabling the detection and quantification of target antigens or proteins in biological samples.

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Lab products found in correlation

Pierce ecl western blotting substrate, by Thermo Fisher Scientific (2 mentions) A5441, by Merck Group (1 mentions) Anti β actin a5441, by Merck Group (1 mentions)

Close spelling variants of this product

Horseradish peroxidase (23 citations) HRP-conjugated antibodies (8 citations) Horseradish peroxidase (HRP) (4 citations) Horseradish peroxidase-conjugated anti-mouse or anti rat antibodies (2 citations)

2 protocols using horseradish peroxidase hrp conjugated anti mouse or anti rat antibodies

1

Western Blot Protein Detection

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Whole cell extracts were separated by 12% SDS-PAGE under reducing conditions and transferred to PVDF membranes. Proteins were detected with antibodies against HA (3F10, Roche; 1:5000) or β-actin (A5441, Sigma Aldrich; 1:5000). Proteins of interest were detected with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rat antibodies (Southern Biotech; 1:5000). Visualization was performed with Pierce ECL western blotting substrate (Thermo Scientific).
López-Sánchez U., Nicolas G., Richard A.C., Maltête D., Charif M., Ayrignac X., Goizet C., Touhami J., Labesse G., Battini J.L, & Sitbon M. (2019). Characterization of XPR1/SLC53A1 variants located outside of the SPX domain in patients with primary familial brain calcification. Scientific Reports, 9, 6776.
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2

Western Blot Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell extracts were separated by 12 % SDS-PAGE under reducing conditions and transferred to PVDF membranes. Protein expression and transfer were monitored with anti-HA tag antibodies (3F10, Roche; 1:5000) or anti-b-actin (A5441, Sigma-Aldrich; 1:5000), followed by horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rat antibodies (Southern Biotech; 1:5000), and visualization with Pierce ECL western blotting substrate (Thermo Scientific).
Anheim M., López-Sánchez U., Giovannini D., Richard A.C., Touhami J., N'Guyen L., Rudolf G., Thibault-Stoll A., Frebourg T., Hannequin D., Campion D., Battini J.L., Sitbon M, & Nicolas G. (2016). XPR1 mutations are a rare cause of primary familial brain calcification. Journal of neurology, 263(8).
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