Twenty-two of the 50 blood samples from patients (17 NSCLC, 5 SCLC) were evaluated for CTCs by using the CellSearch system (Janssen Diagnostics) for direct comparison with our MCA system. Peripheral blood (10 mL) was collected into CellSave Preservative Tubes (Janssen Diagnostics), and measurement with the CellSearch system was performed at GeneticLab Co., Ltd. (Sapporo, Japan).
Cellsearch system
Manufactured by Johnson & Johnson
Sourced in United States
The CellSearch system is a laboratory instrument used for the detection and enumeration of circulating tumor cells (CTCs) in blood samples. It employs immunomagnetic separation and fluorescence microscopy techniques to identify and count rare tumor cells present in the bloodstream. The CellSearch system provides objective and standardized results, enabling healthcare professionals to monitor disease progression and response to treatment.
Automatically generated - may contain errors
Lab products found in correlation
Cellsearch cxc kit, by Johnson & Johnson (3 mentions)
Circulating endothelial cell isolation kit, by Johnson & Johnson (2 mentions)
Cellsave tubes, by Johnson & Johnson (2 mentions)
Celltracks analyzer 2, by Johnson & Johnson (2 mentions)
Cellsave preservative tube, by Johnson & Johnson (2 mentions)
Microvue bap eia kit, by Quidel (1 mentions)
Cellsearch, by Johnson & Johnson (1 mentions)
Cellsave, by Johnson & Johnson (1 mentions)
Cellsearch ctc kit, by Johnson & Johnson (1 mentions)
23 protocols using cellsearch system
1
Comparative CTC Enumeration Methods
2
CellSearch CTC Detection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The CellSearch system (Janssen Diagnostics LLC, Raritan, NJ, USA) was used for the detection and evaluation of CTCs according to the manufacturer's protocol. A blood volume of 7.5 ml was prepared for each sample analysis.
3
CTC Detection using CellSearch System
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of 7.5 ml of EDTA-anticoagulated blood was used for CTC detection using the FDA-approved CellSearch system (Janssen, Beerse, Belgium). Blood samples were processed within 72 h from venipuncture using the CellSearch CXC kit (Janssen
4
Standardized Enumeration of Circulating Endothelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CEC counts were performed by means of the Circulating Endothelial Cell isolation kit (research use only) in combination with the CellSearch system (Janssen Diagnostics LLC, Raritan, NJ, USA), that allows standardization of the whole process of sample collection, cellular selection, monoclonal antibodies labeling, analysis and enumeration of CEC, as previously described. 20 To be scored as a CEC, a CD146 + cell must have a nucleus (DAPI), express CD105, show the morphology of an intact cell, and be negative for CD45. Overall, CEC were defined as CD146 + /CD105 + /DAPI + / CD45 -cells. Counts were expressed as number of CEC per mL of PB.
5
CTC and CEC Enumeration in Metastatic Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CTC and CEC counts were measured in 7.5 and 4 ml of blood using CellSearch System (Janssen Diagnostics) which combines EpCAM immunomagnetic selection followed by anti-cytokeratin staining for CTC detection, and CD146 immunomagnetic selection and CD105 staining for CEC. Blood samples were taken from each patient: (i) before the first cycle (baseline); (ii) before the first docetaxel cycle (cycle 5); (iii) before surgery; and (iv) after surgery before the reintroduction of bevacizumab.
6
Standardized CEC Enumeration in Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CEC counts were performed, within 48 hours from collection, using the Circulating Endothelial Cell isolation kit (research use only) on the CellSearch system (Janssen Diagnostics LLC, Raritan, NJ, USA), allowing to standardize the process of sample collection, cellular selection, monoclonal antibodies labelling, analysis and enumeration of CEC10 (link). CEC were defined as CD146+/CD105+/DAPI+/CD45− cells and their values expressed per mL of PB.
7
Detecting Circulating Tumor Cells in CSF
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Complete methods for this procedure are detailed in Supplementary Methods . In brief, CTCs in CSF from patients with LMD were detected by adapting the CellSearch system (Janssen Diagnostics, Raitan, NJ, USA) using the CELLTRACKS Circulating Melanoma Cell Kit as previously described.11 (link)
8
Circulating Tumor Cells in mCRPC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In eligible patients, CTCs were determined no more than 1 week prior to start of the planned treatment (CTC_1). For all patients, a second CTC determination was planned after 2–3 months (CTC_2). For each CTC determination, blood was collected into CellSave Preservation Tubes (Janssen Diagnostics LLC), and 7.5 mL blood was analyzed per sample. Isolation and enumeration of CTCs were carried out using the Food and Drug Association (FDA)-approved CellSearch system (Janssen Diagnostics LLC), with cell interpretation following the standard CellSearch CTC definition.17 (link) Following previous studies in mCRPC patients6 (link),7 (link) a finding of <5 CTCs/7.5 mL blood was defined as “favorable”.
PSA, total ALP, bone-specific ALP (bone-ALP), and LDH were determined at the same days that the CTC determinations took place. LDH and total ALP were measured on a Cobas® L501 analyzer, while PSA was measured on Cobas L601 analyzer (F Hoffman-La Roche Ltd, Basel, Switzerland). Bone-ALP levels were determined with a MicroVue™ BAP EIA kit (Quidel Corporation, San Diego, CA, USA).
PSA, total ALP, bone-specific ALP (bone-ALP), and LDH were determined at the same days that the CTC determinations took place. LDH and total ALP were measured on a Cobas® L501 analyzer, while PSA was measured on Cobas L601 analyzer (F Hoffman-La Roche Ltd, Basel, Switzerland). Bone-ALP levels were determined with a MicroVue™ BAP EIA kit (Quidel Corporation, San Diego, CA, USA).
9
Circulating Tumor Cell Enumeration
10
Circulating Tumor Cells in Non-Metastatic Breast Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Peripheral blood (7·5 mL) was collected at the time of primary tumor surgery but prior to any surgical manipulation of the primary tumor. Circulating tumor cell status was determined using the CellSearch System® (Janssen Diagnostics, LLC) within 72 h of blood collection. This semi-automated technology uses antibody-coated magnetic beads to enrich blood samples for cells expressing the epithelial-cell-adhesion molecule, labels the nuclei of these enriched cells with the fluorescent dye 4,2-diamidino-2-phenylindole dihydrochloride, and stains the enriched cells using a combination of CK 8,18,19, and CD45 fluorescent antibodies. A semi-automated fluorescence-based microscope system was employed to identify CTCs; nucleated cells were positive for CK and negative for CD45, as described previously [11 (link)]. All results were reviewed by a qualified laboratory technician who was blinded to all patient data. Given the prognostic significance of one or more CTCs in patients with non-metastatic breast cancer, a cutoff of one or more CTCs was considered positive in this study.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!