Log In Sign up
The largest database of trusted experimental protocols

Anti ha apc antibody

Manufactured by BioLegend
Visit Supplier

The Anti-HA APC antibody is a fluorescently labeled antibody that specifically binds to the hemagglutinin (HA) epitope tag. It can be used for the detection and analysis of HA-tagged proteins in various applications.

Automatically generated - may contain errors

Lab products found in correlation

Goat f ab 2 anti human igg pe, by Southern Biotech (2 mentions) Prism 9, by GraphPad (1 mentions) Facscanto 2 flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Anti-HA antibody Anti-HA

2 protocols using anti ha apc antibody

1

Epitope Mapping of Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in epitope mapping studies were titrated against yeast displaying GP38 to adequately calculate EC50s and EC80s for each antibody. Yeast were induced to express non-mutagenized GP38 as noted above. Antibodies were titrated from 100 nM in two-fold, 12-point serial dilutions. Once an OD600 of 0.1 was achieved, the non-mutagenized GP38-expressing yeast were mixed with each antibody dilution and incubated on ice for one hour. Yeast cells were washed two times with PBSF and subsequently stained for 30 minutes on ice with a cocktail of anti-HA APC antibody (Biolegend, Clone: 16B12, dilution 1:100), goat F(ab′)2 anti-human IgG PE (SouthernBiotech, dilution 1:100), and PI (Invitrogen, 1:100 dilution). After staining, samples were run on a FACSCanto II flow cytometer (BD Biosciences). PE MFIs were plotted against antibody concentrations; EC50 and EC80 concentrations were calculated using GraphPad Prism 9.
Shin O.S., Monticelli S.R., Hjorth C.K., Hornet V., Doyle M., Abelson D., Kuehne A.I., Wang A., Bakken R.R., Mishra A., Middlecamp M., Champney E., Stuart L., Maurer D.P., Li J., Berrigan J., Barajas J., Balinandi S., Lutwama J.J., Lobel L., Zeitlin L., Walker L.M., Dye J.M., Chandran K., Herbert A.S., Pauli N.T, & McLellan J.S. (2024). Crimean-Congo Hemorrhagic Fever Survivors Elicit Protective Non-Neutralizing Antibodies that Target 11 Overlapping Regions on Viral Glycoprotein GP38. bioRxiv.
+ Open protocol
+ Expand
2

Screening CCHFV Gc Mutant Library

Check if the same lab product or an alternative is used in the 5 most similar protocols
Displayed wild-type IbAr10200 Gc was used as a control to draw the gates for negative selection of mutant libraries. All staining steps were performed on ice and in the dark when fluorophores were present. Wild-type Gc or Gc mutant libraries were stained with an anti-CCHFV rGn/Gc IgG at 10 nM in PBSF. After washing twice with PBSF, wild-type Gc or Gc mutant libraries were stained with an anti-HA APC antibody (BioLegend, Clone: 16B12, Dilution: 1:100) and Goat F(ab’)2 anti-human IgG PE (SouthernBiotech, Dilution: 1:100). Clones displaying loss-of-binding mutants were sorted and cultured in SC media containing dextrose (see above) for subsequent rounds of selectio.
Fels J.M., Maurer D.P., Herbert A.S., Wirchnianski A.S., Vergnolle O., Cross R.W., Abelson D.M., Moyer C.L., Mishra A.K., Aguilan J.T., Kuehne A.I., Pauli N.T., Bakken R.R., Nyakatura E.K., Hellert J., Quevedo G., Lobel L., Balinandi S., Lutwama J.J., Zeitlin L., Geisbert T.W., Rey F.A., Sidoli S., McLellan J.S., Lai J.R., Bornholdt Z.A., Dye J.M., Walker L.M, & Chandran K. (2021). Protective neutralizing antibodies from human survivors of Crimean-Congo hemorrhagic fever. Cell, 184(13), 3486-3501.e21.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!