Western blot was performed using the following primary antibodies: anti-human Nox2, anti-human Nox4 (BD Biosciences, San Diego, CA, USA), and anti-human β-actin antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA). GSK2795039, a Nox2 inhibitor, was obtained from MedChemExpress (Monmouth Junction, NJ, USA). Anti-human caspase 3 and 9 antibodies (Abcam, Cambridge, MA, USA) and anti-human Mcl-1, Bcl-2, Bim, Noxa antibodies (Cell Signaling Technology, Beverly, MA, USA) were used to evaluate protein expression. N-Benzyloxycarbonyl-Val-Ala-Asp (O-Me) fluoromethyl ketone (Z-VAD-FMK, Sigma, St. Louis, USA), a pan-caspase inhibitor; N-acetyl-L-cysteine (NAC, Cell Signaling Technology), a ROS scavenger; and ABT-263 (Selleck Chemical, Shanghai, China), a Bim specific inhibitor were used to identify the intra-cellular signaling pathway.
N acetyl l cysteine nac
Manufactured by Cell Signaling Technology
Sourced in United States
N-acetyl-L-cysteine (NAC) is a synthetic derivative of the amino acid cysteine. It serves as a precursor to the antioxidant glutathione, which plays a crucial role in cellular defense against oxidative stress.
Automatically generated - may contain errors
Lab products found in correlation
N benzyloxy carbonyl val ala asp ome fluoromethylketone zvadfmk, by Merck Group (1 mentions)
Abt 263, by Selleck Chemicals (1 mentions)
Gsk2795039, by MedChemExpress (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
4 phenylbutyrate 4 pba, by Cell Signaling Technology (1 mentions)
Aicar, by Cell Signaling Technology (1 mentions)
Chloroquine cq, by Cell Signaling Technology (1 mentions)
Trichostatin a tsa, by Cell Signaling Technology (1 mentions)
Mg132, by Cell Signaling Technology (1 mentions)
2 protocols using n acetyl l cysteine nac
1
Investigating Nox2/Nox4 Signaling in Cell Apoptosis
2
Modulation of PTC cell lines
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Human PTC cell lines TPC-1 and K1 were purchased from the University of Colorado Cancer Center Cell Bank. All cells were cultured in RPMI 1640 medium supplemented with 10% FBS (Invitrogen, Carlsbad, CA, USA) at 37 °C in a 5% CO 2 atmosphere. ROS scavenger N-acetyl-L-cysteine (NAC), histone deacetylase HDAC inhibitor Trichostatin A (TSA), AMPK pathway activator AICAR, autophagy inhibitor chloroquine (CQ), ER-stress inhibitor 4-phenylbutyrate (4-PBA), proteasome inhibitor MG-132 were all purchased from Cell Signaling Technology. The work concentration and time were con rmed by instructions combined with experimental requirements.
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