Bmp 2 antibody

The expression of BMP2 in the mouse retina was examined with immuno-fluorescence using BMP2 antibody (Santa Cruz Biotechnology, Dallas, Texas) and isolectin-B4 as a vascular marker (Vector Laboratories, Burlingame, CA). Briefly, frozen retinal sections from normal and diabetic mice were fixed in paraformaldehyde (4%) for 10 min followed by incubation in 3% normal goat serum for 1 h in a humidified container. The sections were then incubated in primary antibody (1:100), isolectin-B4 (15 μg/ml) and NGS (1:50) overnight in a humidified container at 4 °C. In next day the sections were incubated in avidin-conjugated Texas red 25ug/mL (Vector Laboratories, Burlingame, CA) and Oregon green-labeled anti-rabbit antibody (1:500) (Molecular Probes, Eugene, OR). Sections were covered using 4′, 6diamidino-2-phenylindole (DAPI) mounting medium (Vector Laboratories, Burlingame, CA), and images were obtained with confocal microscopy (LSM 510; Carl Zeiss, Thornwood, NY).