A total of 8 µm cryosections were rehydrated for 5 min in PBS and immersed for 5 min in a 60% isopropanol solution before being stained for 15 min in a freshly prepared 0.3% (w/v) Oil Red O solution in 60% isopropanol. The stained sections were then rinsed in 60% isopropanol and counterstained using hematoxylin (#H-3401-500, Vector Laboratories) for 2 min before being mounted in an aqueous mounting medium.
H 3401 500
Manufactured by Vector Laboratories
The H-3401-500 is a laboratory equipment product manufactured by Vector Laboratories. It serves as a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
Hematoxylin, by Vector Laboratories (1 mentions)
3 3 diaminobenzidine (dab), by Merck Group (1 mentions)
Dmrbe, by Leica (1 mentions)
E9884, by Merck Group (1 mentions)
C2404, by Merck Group (1 mentions)
L182 50, by Harvard Bioscience (1 mentions)
D5905, by Merck Group (1 mentions)
C9368, by Merck Group (1 mentions)
2 protocols using h 3401 500
1
Oil Red O Staining of Lipid Droplets
2
Immunohistochemical Staining of Liver Tissue
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Liver tissue sections were dewaxed in xylene (9713.2; Carl Roth) and rehydrated in gradient ethanol (K928.4; Carl Roth). For antigen retrieval, slides incubated with 1 mM EDTA (E9884, pH 8.4; Sigma-Aldrich) or citrate acid buffer (C2404, pH 6.0; Sigma-Aldrich) were heated by microwave (15 s full power, 1000 W, 45 s shutdown, 10 cycles). Following cooling at room temperature for 30 min, the slides were incubated in blocking peroxide (S200389-2; Dako) for 30 min to reduce nonspecific staining. Subsequently, primary antibodies were added overnight at 4°C. The next day, following washing with PBS (L182-50; Biochrom) three times, sections were incubated with horseradish peroxidase-labeled secondary antibodies for 45 min. Visualization was achieved with 3,3-diaminobenzidine (D5905; Sigma-Aldrich). Counterstaining was performed with hematoxylin (H-3401-500; Vector Laboratories). Finally, all sections were dehydrated and mounted with malinol mounting medium (C9368; Sigma-Aldrich). Images were taken under a microscope (DMRBE; Leica).
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